| 酸马奶中乳杆菌Lb.casei.Zhang和ZL12-1的16S rDNA基因序列及聚类分析 |
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| 引用本文： | 乌日娜,张和平,孟和毕力格.酸马奶中乳杆菌Lb.casei.Zhang和ZL12-1的16S rDNA基因序列及聚类分析[J].中国乳品工业,2005,33(6):4-9. |
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| 作者姓名： | 乌日娜 张和平 孟和毕力格 |
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| 作者单位： | 教育部乳品生物技术与工程重点实验室,内蒙古农业大学食品科学与工程学院,内蒙古,呼和浩特,010018 |
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| 基金项目： | 教育部科学技术研究重点项目(205080)，教育部春晖计划项目(Z2004-2-15009) |
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| 摘 要： | 利用遗传学方法对分离自内蒙古锡林郭勒盟牧区采集的酸马奶样品中的乳杆菌Lb．casei．Zhang和ZL12-1的16S rDNA扩增与测序．并将结果与同属的乳杆菌作同源性分析，建立系统发育树，以求更准确地确定其归属，为益生菌的筛选奠定基础。结果表明，菌株Lb.casei.Zhang标准菌株Lb．casei ATCC334^T同源性为100％，菌株zLl2—1与标准Lb.gallinanum ATCC 33199^T的同源性为98％。结合系统发育树及16S rDNA的部分序列分析结果，将Lb.casei.Zhang判定为Lb．caseissubsp.casei.ZL12-1鉴定为Lb.gallinanum，这与传统分类方法所得鉴定结果一致。
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| 关 键 词： | 乳杆菌 16S rDNA同源性 系统发育树 酸马奶 |
| 文章编号： | 1001-2230(2005)06-0004-06 |
| 修稿时间： | 2005年3月18日 |
16S rDNA sequence and cluster analysis of Lb.casei. Zhang and ZL12-1 isolated from Koumsis |
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| WU Ri-na,ZHANG He-ping,MENGHE Bilige.16S rDNA sequence and cluster analysis of Lb.casei. Zhang and ZL12-1 isolated from Koumsis[J].China Dairy Industry,2005,33(6):4-9. |
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| Authors: | WU Ri-na ZHANG He-ping MENGHE Bilige |
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| Abstract: | To further differentiate Lb.casei.Zhang and ZL12-1 isolated from home-made koumiss in Inner Mongolia, the genes of 16S rDNA from them were amplified in vitro and sequenced. Then a phylogenetic dendrogram was constructed by comparing these two sequences with other 16S rDNA sequences of Lactobacillus. Results indicated that the homology of Lb.casei.Zhang with Lb.casei ATCC 334T was 100%, and the homology of ZL12-1 with Lb.gallinarum ATCC 33199T was 98%. Combined with analysis of phyolgenetic dendrogram and partial sequences of 16S rDNA, Lb.casei.Zhang was classified as Lb.casei subsp.casei , ZL12-1 belonged to Lb.gallinarum. The result was as same as that of traditional classification before. |
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| Keywords: | Lactobacillus 16S rDNA homology phylogenetic dendrogram koumiss |
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