| 高效液相色谱-串联质谱法检测河豚毒素的 方法研究 |
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| 引用本文： | 吴佳俊,黄文雯,肖陈贵,岳振峰,林群声,陈 荔.高效液相色谱-串联质谱法检测河豚毒素的 方法研究[J].食品安全质量检测技术,2014,5(11):3529-3536. |
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| 作者姓名： | 吴佳俊 黄文雯 肖陈贵 岳振峰 林群声 陈 荔 |
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| 作者单位： | 深圳市海洋生物多样性可持续利用重点实验室,深圳市海洋生物多样性可持续利用重点实验室,深圳出入境检验检疫局食品检验检疫技术中心,深圳出入境检验检疫局食品检验检疫技术中心,深圳市海洋生物多样性可持续利用重点实验室,深圳市海洋生物多样性可持续利用重点实验室 |
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| 摘 要： | 目的 创新性地建立了一种用高效液相色谱－串联质谱法检测水产品中的河豚毒素的方法。方法 取适量样品,在0.03M乙酸溶液中均质后超声提取样品中的河豚毒素。粗提液低速离心,取上清液加入等量甲醇充分混匀后进行高速离心。高速离心后上清液过0.22μm滤膜后待测。采用Waters ACQUITY UPLC BEH Amide(2.1×100mm,1.7μm)色谱分析柱,含0.1%甲酸的MilliQ水及乙腈溶液为流动相,电喷雾正离子模式进行分析。结果 河豚毒素在5-1000 ng/mL的范围内线性关系良好,相对回收率为85.8%~93.2%,绝对回收率为47.8%~48.9%,检出限为0.4ng/mL,定量限为1 ng/mL。 结论 与以往检测TTX的LC-MS/MS方法相比,新建方法操作简单、快速、准确、灵敏并且实验成本低,为水产品中TTX的定量检测提供了有效的实用技术手段。
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| 关 键 词： | 高效液相色谱－串联质谱法 河豚毒素 水产品 亲水性色谱分析柱 |
| 收稿时间： | 2014/10/8 0:00:00 |
| 修稿时间： | 2014/11/6 0:00:00 |
Determination of tetrodotoxin by high performance liquid chromatogra-phy-tandem mass spectrometry |
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| WU Jia-Jun,HUANG Wen-Wen,XIAO Chen-Gui,YUE Zhen-Feng,LAM Kwan-Sing and CHAN Lai.Determination of tetrodotoxin by high performance liquid chromatogra-phy-tandem mass spectrometry[J].Food Safety and Quality Detection Technology,2014,5(11):3529-3536. |
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| Authors: | WU Jia-Jun HUANG Wen-Wen XIAO Chen-Gui YUE Zhen-Feng LAM Kwan-Sing and CHAN Lai |
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| Affiliation: | Shenzhen Key Laboratory for Sustainable Use of Marine Biodiversity,Research Centre for the Oceans and Human Health,City University of Hong Kong Shenzhen Research Institute,Shenzhen Key Laboratory for Sustainable Use of Marine Biodiversity,Research Centre for the Oceans and Human Health,City University of Hong Kong Shenzhen Research Institute,Key Laboratory of Detection Technology R D on Food Safety,Food Inspection and Quarantine Center,Shenzhen Entry-Exit Inspection and Quarantine Bureau,Key Laboratory of Detection Technology R D on Food Safety,Food Inspection and Quarantine Center,Shenzhen Entry-Exit Inspection and Quarantine Bureau,Shenzhen Key Laboratory for Sustainable Use of Marine Biodiversity,Research Centre for the Oceans and Human Health,City University of Hong Kong Shenzhen Research Institute,Shenzhen Key Laboratory for Sustainable Use of Marine Biodiversity,Research Centre for the Oceans and Human Health,City University of Hong Kong Shenzhen Research Institute |
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| Abstract: | |
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| Keywords: | high performance liquid chromatography-tandem mass spectrometry tetrodotoxin aquatic products HILIC column |
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