化学发光免疫方法在食品安全检测中的应用及展望

近年来,随着各种食品问题的突发事件频繁发生,食品安全已经引起了社会各方面的广泛关注,传统的食品安全检测技术因操作复杂,耗时长,成本高等特点,无法满足当前检测需求,因此建立快速、灵敏、准确检测手段成为保障食品安全的研究重点。化学发光免疫分析(CLIA)具有高的灵敏度、特异性好、分析速度快、简便的操作优势,受到越来越多的食品安全领域人员青睐。CLIA能够很灵敏的对有毒有害物质进行区别,能检测出不同分子量大小的抗原、半抗原和抗体,同时还能应用于核酸探针,它目前被广泛用于食品、医疗和环境等领域,根据近五年的文献报道,简单概述了CLIA技术,并从农药残留、兽药残留、违禁添加物、病原微生物以及生物毒素等方面在食品安全检测中的作了详细综述,对该技术进行了展望。

Application and prospect of chemiluminescence immunoassay in food safety detection

ABSTRACT: In recent years, with the frequent occurrence of various food problems, food safety has attracted wide attention in all aspects of society. The traditional food safety detection technology can can''t the current detection needs because of its complex operation, long time consuming and high cost.Therefore, the establishment of rapid, sensitive and accurate detection means has become the focus of food safety research. Chemiluminescent immunoassay (CLIA) has the advantages of high sensitivity, good specificity, fast analysis speed and easy operation, and is favored by more and more people in the field of food safety. CLIA is sensitive to distinguish between toxic and harmful substances, can detect different molecular weight of antigen, haptens and antibodies, and can also be used in nucleic acid probes, which are widely used in food, medicine and the environment. According to the literature reports in the past five years, briefly summarized the CLIA technology, and made a detailed review on the food safety detection in terms of pesticide residues, veterinary drug residues, prohibited additives, pathogenic microorganisms and biological toxins and made a prospect of this technology.