高压液相色谱法测定纸质食品接触材料中亚甲基双硫氰酸酯的迁移量

目的 建立纸质食品接触材料中亚甲基双硫氰酸酯迁移量的高压液相色谱测定方法。方法 食品模拟物(水、3%乙酸-水溶液、10%乙醇-水溶液和橄榄油)中的亚甲基双硫氰酸酯通过高压液相色谱进行分离, 采用二极管阵列检测器进行测定。水基食品模拟物直接进样, 油基食品模拟物经乙腈萃取后进样, 外标法定量。结果 3种水基食品模拟物中, 亚甲基双硫氰酸酯在0.5~50 mg/L范围内线性关系良好, 定量下限为0.5 mg/L, 平均回收率为88.2%~97.4%, 相对标准偏差为0.44%~6.54%; 油基食品模拟物中, 亚甲基双硫氰酸酯在1.0~20 mg/kg范围内线性关系良好, 定量下限为1.0 mg/kg, 平均回收率为99.3%~104.7%, 相对标准偏差为0.75%~3.15%。结论 该方法准确、简便、快速, 可用于纸质食品接触材料中亚甲基双硫氰酸酯迁移量的实际检验工作。

Determination of methylene dithiocyanate migration from food contact paper materials by high pressure liquid chromatography

Objective An analytical method for the detection of methylene dithiocyanate migration from food contact paper materials by high pressure liquid chromatography was established. Methods Methylene dithiocyanate migrated into the food simulants (water, 3% aqueous acetic acid solution, 10% aqueous ethanol solution and refined olive oil) was separated and detected by high pressure liquid chromatography coupled with diode array detector. The water-based food simulants were subjected to instrumental analysis directly and the oil-based food simulantwas extracted by acetonitrile and then analyzed and quantitated with external standard method. Results This method showed a good linearity for methylene dithiocyanate in the range of 0.5~50 mg/L in the 3 water-based food simulants with the limit of quantification of 0.5 mg/L. The mean recoveries at three spiked levels were in the range of 88.2%~97.4% with relative standard deviations of 0.44%~6.54%. Methylene dithiocyanate exhibits a good linearity in the range of 1.0~20 mg/kg with the limit of quantification of 1.0 mg/kg. The mean recoveries at 3 spiked levels were between 99.3% and 104.7% with relative standard deviations of 0.75%~3.15%. Conclusion The method is accurate, simple, and rapid, and it can be used for the determination of methylene dithiocyanate in food contact paper materials.