不同仪器检测方法及标准品对保健食品中茶多酚含量测定的影响

目的以茶多酚及没食子酸为标准品,分别采用紫外分光光度法及高效液相色谱法测定保健食品中茶多酚含量。方法通过对市场上部分宣称含茶多酚的保健食品进行测定,探讨不同仪器检测方法、不同标准物质对保健食品中茶多酚含量的影响。结果高效液相色谱法测定的结果均低于紫外分光光度法,其中紫外分光度法测定中,以茶多酚为标准品测定结果低于以没食子酸为标准品的结果。结论测定保健食品样品中茶多酚含量时,应根据产品的原辅料及目的进行仪器方法及标准品的选择。

Effects of different instrument testing methods and standard substances on determination of tea polyphenols in health food

Objective To establish methods for determination of tea polyphenols in health food by high performance liquid chromatography (HPLC) and ultraviolet (UV) spectrophotometry using tea polyphenols and gallic acid as standards. Methods Through the detection of the part of health food which declared containing tea polyphenols in the market, the effects of different instrument testing methods and standard substances on determination of tea polyphenol in health food were analyzed. Results The results by HPLC were lower than those by UV spectrophotometry. The results using tea polyphenols as standard substances were lower than those using gallic acid in UV spectrophotometry. Conclusion The selection of instrument testing methods and standard substances shall be carried out according to the product of raw materials and purpose in the determination of tea polyphenols in health food.