超高效液相色谱-串联质谱法测定功能饮料中的牛磺酸和赖氨酸

目的 建立超高效液相色谱-串联质谱方法测定功能饮料中牛磺酸和赖氨酸的分析方法。方法 采用衍生化的方法，将稀释之后的饮料样品中的牛磺酸和赖氨酸与苯甲氧羰酰琥珀酰亚胺在碱性条件下进行反应，之后应用超高效液相色谱-串联质谱测定衍生化后的化合物的含量。结果 牛磺酸和赖氨酸分别衍生之后的化合物在1-200 ng/mL的浓度范围内线性良好，牛磺酸的检出限为20 pg/mL，定量限为50 pg/mL，赖氨酸的检出限为100 pg/mL，定量限为250 pg/mL。两种物质在低、中、高三个浓度的加标回收率均在90-110%之间，相对标准偏差(RSD)均小于10%。结论 该方法样品前处理非常简单，衍生化反应步骤简便，灵敏度高，重复性好，可用于快速准确的测定饮料中的牛磺酸和赖氨酸的含量。

Determination of taurine and lysine in functional drinks by ultra performance liquid chromatography-tandem mass spectrometry

Objective To establish a method for the determination of taurine and lysine in functional drinks by ultra performance liquid chromatography-tandem mass spectrometry. Methods Taurine and lysine in diluted beverage samples were reacted with benzoxycarbonyl succinimide under alkaline conditions by derivatization method, and then the content of derivatized compounds was determined by ultra performance liquid chromatography-tandem mass spectrometry. Results The compounds derived from taurine and lysine had good linearities in the range of 1?200 ng/mL, the limit of detection of taurine was 20 pg/mL, and its limit of quantification was 50 pg/mL; the limit of detection of lysine was 100 pg/mL, and its limit of quantification was 250 pg/mL. The recoveries of the 2 kinds of substances at low, medium and high concentrations were 92.77%?107.59%, and the relative standard deviations were less than 10% (n=6). Conclusion This method has the advantages of simple sample preparation steps, high detection sensitivity and good repeatability, which can be used for the rapid and accurate determination of taurine and lysine in functional drinks