| 有机磷农药多残留检测化学发光标记物的 合成及鉴定 |
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| 引用本文： | 刘涛,孟 超,姚 鑫,李景娜,程 燕,刘晓宇. 有机磷农药多残留检测化学发光标记物的 合成及鉴定[J]. 食品安全质量检测学报, 2014, 5(5): 1365-1370 |
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| 作者姓名： | 刘涛 孟 超 姚 鑫 李景娜 程 燕 刘晓宇 |
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| 作者单位： | 华中农业大学食品科技学院,华中农业大学食品科技学院; 环境食品学教育部重点实验室,华中农业大学食品科技学院,华中农业大学食品科技学院,华中农业大学食品科技学院,华中农业大学食品科技学院 |
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| 基金项目： | 国家自然科学基金项目(31171694)、湖北省研究与开发计划项目(2010BBB022)、中央高校基本科研业务费专项(2011PY094)、华中农业大学国家级大学生创新创业训练计划项目(20130504035) |
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| 摘 要： | 目的 以O,O-二乙基硫代磷酰氯和鲁米诺为原料, 合成一种新型的化学发光标记物, 用于后续的二乙氧基类有机磷农药多残留化学发光免疫法检测。方法 取鲁米诺和O,O-二乙基硫代磷酰氯溶于二氯甲烷中, 以三乙胺作为缚酸剂, 冰浴下反应8 h后分出有机层, 产物经柱层析纯化, 即得到化学发光标记物。结果 产物经紫外、红外鉴定, 实验结果表明反应产物即为所需的化学发光标记物, 产物在BPCL上进行发光特性测定, 表现出较强的发光能力, 最大发光强度达到9000 mV。结论 本方法合成的化学发光标记物具有较强的发光能力, 能用于后续的二乙氧基类有机磷农药多残留的化学发光免疫检测。
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| 关 键 词： | O,O-二乙基硫代磷酰氯 鲁米诺 化学发光标记物 鉴定 |
| 收稿时间： | 2014-04-11 |
| 修稿时间： | 2014-05-14 |
Synthesis and identification of chemiluminescent markers used in the multi-residue determination of organophosphorus pesticides |
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| LIU Tao,MENG Chao,YAO Xin,LI Jing-N,CHENG Yan and LIU Xiao-Yu. Synthesis and identification of chemiluminescent markers used in the multi-residue determination of organophosphorus pesticides[J]. Journal of Food Safety & Quality, 2014, 5(5): 1365-1370 |
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| Authors: | LIU Tao MENG Chao YAO Xin LI Jing-N CHENG Yan LIU Xiao-Yu |
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| Affiliation: | College of Food Science and Technology, Huazhong Agricultural University,College of Food Science and Technology, Huazhong Agricultural University; Key laboratory of Environment Correlative Dietology, Ministry of Education,College of Food Science and Technology, Huazhong Agricultural University,College of Food Science and Technology, Huazhong Agricultural University,College of Food Science and Technology, Huazhong Agricultural University and College of Food Science and Technology, Huazhong Agricultural University |
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| Abstract: | Objective Chemiluminescent markers were synthesized using O,O-diethylthiophosphoryl chloride and luminol for the multi-residue determination of organophosphorus pesticides. Methods Luminol and O, O-diethyl phosphoryl chloride were added to dichloromethane under ice-cooling with triethylamine as acid binding agent. After 8 h, the organic layer was separated and purified by column chromatography. Results The product was the chemiluminescent markers after indentation using UV and IR. It had a strong luminescent ability on the BPCL, and the maximum luminous intensity reached 9000 mV. Conclusion The chemiluminescent markers synthesized by this method had a strong luminescence capability, and it could be used in the multi-residue determination of organophosphorus pesticides. |
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| Keywords: | O,O-diethylthiophosphoryl chloride luminol chemiluminescent markers identification |
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