基于网络药理学分析红芪免疫调节的作用机制

目的:运用网络药理学方法探讨红芪药理作用的物质基础和作用机制。方法:借助中药系统药理学数据库与分析平台(TCMSP)检索红芪的化学成分和相关作用靶点。进而采用Cytoscape3.6.1、STRING11.0软件构建化合物-靶点网络和蛋白互作(PPI)网络、运用DAVID6.7在线数据库进行基因本体(GO)功能富集分析和基于京都基因与基因组百科全书(KEGG)生物通路富集分析,研究红芪的作用机制。结果:通过筛选得出20 个化合物和225 个相关靶点。PPI网络包含36 个靶点,关键靶点有NOS3、ESR1、HSP90AA1等。基因本体条目27 个,其中生物过程相关条目22 个,分子功能相关条目3 个,细胞组成相关条目2 个。京都基因与基因组百科全书通路8 条,涉及钙离子信号通路、孕酮介导的卵母细胞成熟信号通路、癌症信号通路、神经活性配体-受体相互作用信号通路、前列腺癌信号通路、卵母细胞减数分裂信号通路、血管平滑肌收缩信号通路和细胞周期信号通路。结论:本研究初步阐明了红芪药理作用的分子机制,为进一步深入探讨其作用机制提供参考。

Mechanism of Hedysari radix on immunoregulation based on network pharmacology

Objective To study material basis and mechanism of Hedysari radix on immunoregulation based on network pharmacology. Methods The chemical components and corresponding targets related of Hedysari radix were searched from the traditional Chinese medicine systems pharmacology (TCMSP) database and analysis platform. The compound-target network and the protein-protein interaction (PPI) network were established by Cytoscape 3.6.1 and STRING 11.0 software, and the gene ontology (GO) functional enrichment analysis and the Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis were made by DAVID 6.7 software to explore the mechanism of Hedysari radix. Results Through TCMSP, 43 kinds of compounds were identified, 20 kinds of active compounds were screened and 225 kinds of potential targets were predicted. The PPI network contains 36 kinds of targets, and there were 27 kinds of GO entries, including 22 kinds of biological process entries, 3 kinds of molecular function entries and 2 kinds of cell composition related items. Besides, there were 8 kinds of KEGG pathways. Conclusion Several chemical components of Hedysari radix may be combined with PTGS, PRKACA, ADRB2, RXRA, HSP90 and so on to play an immunomodulatory role. Hedysari radix has the characteristics of multiple components, multiple targets and multiple pathways for immunoregulation, and has potential therapeutic effects on cardiac cerebrovascular disease, inflammation, cancer, etc. The results of this study may provide a theoretical basis and scientific basis for the mechanism of immune regulation of Hedysari radix.