QuEChERS净化超高效液相色谱法快速测定 豆制品中的9种工业染料

目的建立了豆皮、豆腐、腐竹及豆干4种豆制品中的9种工业染料的检测方法。方法以Qu ECh ERS方法作为前处理技术,以超高效液相色谱技术作为检测技术,对方法的有效性进行了验证,并对实际样品进行检测。结果以空白的4种豆制品为基质,分别在3个水平进行加标实验,回收率分别在71.5%~126.4%、74.0%~106.5%、86%~124.5%和76.7%~107.5%之间。RSD(n=5)分别在1.9%~8.5%、1.8%~8.1%、1.8%~7.0%之间和1.7%~8.0%之间。各工业染料的检出限(S/N=3)在0.05~0.2 mg/kg之间,定量限(S/N=10)在0.15~0.6 mg/kg之间。结论该方法操作简单,稳定性好,适于在实际检测工作中推广应用。

Determination of 9 kinds of dyes in bean products by ultra performance liquid chromatography with QuEChERS cleanup

Objective To establish a method for the detection of 9 kinds of industrial dyes in Yuba, tofu, beancurd sticks,and dried tofu . Methods The validity of the method was validated, which used to the actual sample, with QuEChERS as a pretreatment technology and ultra-high performance liquid chromatography as detection method. Results Blank bean products material were used as matrixes, and spiking experiments were carried out in high, medium and low level, respectively. Recoveries were between 71.5% to 126.4%, 74.0% to 106.5%, 86% to 124.5% and 76.7%~107.5%. RSD (n=5) were between 1.9% to 8.5%, 1.8% to 8.1%, 1.8% to 7.0% and 1.7% to 8.0%, the limits of detection (S/N=3) were between 0.05~0.2 mg/kg and the limit of quantification (S/N=10) was between 0.15~0.6 mg/kg. Conclusion The method is simple to operate with a good stability and is suitable for the application in daily testing work.