具有金属螯合活性的蚕豆蛋白酶解物的制备

目的探究蚕豆蛋白酶解物的金属螯合活性,研究其金属螯合活性与其抗氧化活性的关系。方法分别采用碱性蛋白酶、中性蛋白酶、木瓜蛋白酶对蚕豆蛋白进行酶解,并测定其水解物的抗氧化活性与金属螯合活性,选用碱性蛋白酶为酶解蚕豆蛋白制取金属螯合肽的最适酶,以酶解产物的水解度、抗氧化活性及金属螯合活性为测定指标获得合适的水解条件。结果 3种蛋白酶的蚕豆蛋白酶解产物都有金属螯合活性和抗氧化活性,碱性蛋白酶为酶解蚕豆蛋白的最适酶,最适酶解时间为4 h时,得到的酶解产物金属离子螯合率为88.22%,抑制羟自由基能力为220.70 U/mg,总还原力为0.03 U/mg。结论蚕豆蛋白酶解物具有一定的金属离子螯合活性与抗氧化活性,水解度对蚕豆蛋白酶解物的金属离子螯合活性及抗氧化活性有明显的影响,蚕豆蛋白酶解物的金属螯合活性与总还原力及抑制羟自由基能力呈现显著的正相关性,相关系数分别为0.925、0.968(P0.01)。

Preparation of metal chelating peptides from broad bean protein by enzymatic hydrolysis

In order to investigate the metal-chelating abilities of broad bean protein hydrolysates, broad bean protein was hydrolyzed with three proteases (papain protease, neutral protease and alcalase). The hydrolysates were found to have the metal-chelating ability and antioxidant activities, in which the alcalase gave the best effect. To evaluate appropriate hydrolysis condition, metal-chelating abilities, degree of hydrolysis (DH) and the antioxidant activities of broad bean protein hydrolysates during hydrolysis with alcalase were tested. The broad bean protein hydrolysates hydrolyzed for 4 h were shown 88.22% of Fe₂₊chelating rate, 220.70 U/mg of hydroxylSradical scavengingSactivity, 0.03 U/mg of reducingSpower. Furthermore, the effect of DH on the metal-chelating abilities and antioxidant activities of hydrolysates were investigated, the results indicated that the metal-chelating abilities and the antioxidant activities of broad bean protein hydrolysates were positively affected by theSDH. In addition, the metal-chelating abilities of broad bean protein hydrolysates were positively correlated with the 2 indicators of antioxidant activity(r: 0.925 or 0.968).