基于金纳米簇与纳米金的荧光内滤效应检测脂肪酶活性

目的 建立一种基于吐温80自氧化作用催化纳米金形成与荧光内滤机理，相对简单、快速的脂肪酶活性检测方法。方法 使用金纳米簇作为荧光基团，纳米金作为吸收剂，基于荧光内滤机理测定脂肪酶活力。设置对照试验对吐温80浓度、氯金酸浓度、温度、pH等反应条件进行优化，探索最适条件下脂肪酶水解反应时间及吐温80还原纳米金的时间并对反应体系进行抗干扰检测，验证该方法的可行性。结果 当脂肪酶酶活力在13.5~15092.0 U/L时，荧光强度的差值随脂肪酶活力的升高而增加，其线性相方程为y=0.06468x-12.1520，相关系数为0.9977，脂肪酶活力的检出限为2.34 U/L（信噪比为3）。结论 该方法操作相对简单、快速，灵敏度高，具有实用性，适合应用于脂肪酶活力的测定。

Detection of lipase activity based on fluorescence internal filtration effect of gold nanoclusters and gold nanoparticles

Objective To establish a relatively simple and rapid detection method for lipase activity based on the formation of gold nanoclusters catalyzed by Tween 80 self-oxidation and the fluorescence inner filter mechanism. Methods Using gold nanoclusters as fluorophores and gold nanoparticles as absorbent, the lipase activity was determined based on the fluorescence inner filter mechanism. A control experiment was set up to optimize the reaction conditions such as Tween 80 concentration, chloroauric acid concentration, temperature, and pH. The time of lipase hydrolysis reaction under optimal conditions and the time of Tween 80 reducing gold nanoparticles were explored. The anti-interference detection of the reaction system was performed to verify the feasibility of the method. Results When the lipase activity was 13.5~15092.0 U/L, the difference of fluorescence intensity increased with the increase of lipase activity. The linear phase equation was y=0.06468x-12.1520, and the correlation coefficient was 0.9977. The detection limit of lipase activity was 2.34 U / L ( signal-to-noise ratio was 3 ). Conclusion The method is relatively simple, rapid, sensitive and practical, which is suitable for the determination of lipase activity.