蜜环菌菌丝与菌索活性成分含量研究

测定蜜环菌(Armillaria mellea)菌丝、菌索两种形态总多糖、总蛋白、总三萜、麦角甾酮、麦角甾醇、腺苷的含量差异,为蜜环菌不同形态的综合开发利用提供理论依据。利用紫外-可见分光光度法测定总三萜、总多糖及总蛋白含量。利用高效液相色谱法测定麦角甾酮、麦角甾醇、腺苷成分含量。蜜环菌菌丝总三萜含量31.14mg/g、总多糖含量9.39mg/g、总蛋白含量101.40mg/g、麦角甾酮含量4.89μg/g、麦角甾醇含量944.48μg/g、腺苷含量78.54μg/g；蜜环菌菌索总三萜含量3.43mg/g、总多糖含量21.71mg/g、总蛋白含量40.72mg/g、麦角甾酮含量5.91μg/g、麦角甾醇含量357.69μg/g、腺苷含量83.58μg/g。蜜环菌菌丝总三萜、总蛋白、麦角甾醇含量优于菌索,且具有显著性差异；蜜环菌菌索总多糖含量、麦角甾酮含量优于菌丝,含量差异明显；两者腺苷含量相似。蜜环菌菌丝与菌索之间的成分含量差异显著,提示不同形态可以分别开发利用。

Study on the active ingredient content of mycelium and rhizomorphs of Armillaria mellea

Objective To study the differences in the content of Armillaria mellea mycelium and rhizomorphs, total polysaccharides, total proteins, total triterpenes, ergosterone, ergosterol and adenosine, provide a theoretical basis for the comprehensive development and utilization. Methods The content of total triterpenes, total polysaccharides and total protein was determined by ultraviolet-visible spectrophotometry. The content of ergosterone, ergosterol and adenosine was determined by high performance liquid chromatography. Results There were significant differences in the content of most active components between Armillaria mellea mycelium and rhizomorphs. The total triterpenes, total protein and ergosterol showed that the content of mycelium was better than that of rhizomorphs, and there was a significant difference (P<0.05); the total polysaccharides content and ergosterone content of rhizomorphs were better than mycelium, and the content difference was obvious; the content of adenosine was similar between the two. Conclusion For the medicinal use and development of Armillaria mellea and the development of health food, it is necessary to develop rationally according to specific plans to achieve rational utilization of resources. The research results can lay a theoretical foundation for future comprehensive utilization and development.