壳寡糖抑菌及抗肿瘤活性研究

目的 初步探讨壳寡糖的抑菌及抗肿瘤活性。方法 采用牛津杯法研究壳寡糖的抑菌活性；采用动物实验法研究壳寡糖的抗肿瘤活性，选取造模成功的50只昆明种雄性小鼠，随机分为5组，分别为模型组、阳性对照组(20 mg/kg)以及壳寡糖高剂量组(300 mg/kg)、中剂量组(150 mg/kg)、低剂量组(100 mg/kg)。结果 牛津杯法证实壳寡糖质量浓度较低时(10、20 mg/mL)，壳寡糖对大肠杆菌、沙门氏菌和绿脓杆菌均无抑菌作用；壳寡糖质量浓度在50~400 mg/mL时，对这3种菌均有抑菌作用，且随壳寡糖质量浓度的升高，抑菌能力逐渐增强；但壳寡糖质量浓度在10~400 mg/mL时，对金黄色葡萄球菌无抑菌作用。动物实验证实壳寡糖能够剂量依赖性的降低小鼠H22瘤体重量，提高小鼠胸腺指数和脾脏指数，其中壳寡糖中、高剂量可显著抑制小鼠H22肿瘤增长(P<0.05)，抑瘤率分别为31.65%和44.30%。结论 壳寡糖对大肠杆菌、沙门氏菌和绿脓杆菌具有良好的抑菌活性以及抑制小鼠移植性肿瘤H22生长的作用。

Research on antibacterial and antitumor activity of chitooligosaccharides

Objective To study on the antibacterial and antitumor activity of chitooligosaccharides. Methods The antibacterial activity of chitooligosaccharides was determined by Oxford cup method and antitumor activity was determined by mice test. 50 Kunming male mice with successful modeling were randomly divided into 5 groups, including model control group, positive control group (20 mg/kg), and high, medium and low dose groups of chitooligosaccharides: 300, 150 and 100 mg/kg. Results The Oxford cup method confirmed that chitooligosaccharides had no antibacterial activity against Escherichia coli, Salmonella and Pseudomonas aeruginosa at low concentrations (10, 20 mg/mL). When the concentration of chitooligosaccharides was 50~400 mg/mL, they had antibacterial activities against these three bacteria, and the antibacterial ability gradually increased with the increase of chitooligosaccharide concentration. However, chitooligosaccharides had no antibacterial activity against Staphylococcus aureus at the concentration of 10~400 mg/mL. Animal experiments showed that chitooligosaccharides could reduce the tumors weight, improve thymus index and spleen index of mice, and the higher dose, the better effect. The medium and high dose groups of chitooligosaccharides could significantly inhibit the tumors growth (P<0.05), and the tumor inhibition rates were 31.65%, 44.40%, respectively. Conclusion Chitooligosaccharides has good antibacterial activity against Escherichia coli, Salmonella, Pseudomonas aeruginosa and inhibits the growth of transplanted tumor H22 in mice.