龙安柚花毒理学安全性评价

目的 评估龙安柚花的食用安全性。方法 按现行食品安全国家标准进行急性经口毒性试验、细菌回复突变（Ames）试验、哺乳动物红细胞微核试验、体外哺乳类细胞染色体畸变试验、90 d经口毒性试验和致畸试验，并进行统计分析。结果 在本试验条件下，在SD大鼠和KM小鼠摄入龙安柚花后均未表现出急性经口毒效应，急性经口半数致死量（lethal dose 50, LD50）大于10 g/(kg·BW)，该花属实际无毒级；龙安柚花未引起TA97a、TA98、TA100、TA102、TA1535菌种发生回复突变；哺乳动物红细胞微核试验与体外哺乳类细胞染色体畸变试验结果均为阴性；90 d经口毒性试验中，与对照组相比，各剂量组各性别动物的体重、摄食量、食物利用率、血液学、血生化和组织病理学检查均未见毒效应，龙安柚花未观察到有害作用剂量（no observed adverse effect level, NOAEL）为8 g/(kg·BW)；未观察到致畸作用。结论 龙安柚花属实际无毒级，本试验条件下龙安柚花无急性毒性、遗传毒性、致畸性和亚慢性毒性。

Toxicological safety evaluation on the flower of Citrus grandis var. Longanyou

Objective To evaluate the toxicity on the flower of Citrus grandis var. Longanyou. Methods Acute oral toxicity test, acterial revert mutation (Ames) test, mammalian erythrocyte micronucleus test, chromosome aberration test of mammalian cells in vitro, 90 d oral toxicity test and teratogenicity test were conducted according to the current national food safety standards. Statistical analysis was conducted. Results Under the condition of this experiment, there was no acute oral toxic effect on the flower of Citrus grandis var. Longanyou in SD rats and KM mice, and the acute lethal dose 50 (LD50) was greater than 10 g/(kg·BW), was the actual non-toxic level. No reverse-mutation of TA97a, TA98, TA100, TA102, TA1535 was induced by the flower of Citrus grandis var. Longanyou. The results were negative in mammalian erythrocyte micronucleus test as well as chromosome aberration test of mammalian cells in vitro. In the 90-day oral toxicity test, compared with the control group, the weight, food intake, food utilization rate, hematology, blood biochemistry and histopathology of animals of all sexes in each dose group did not manifest toxic effects. No observed adverse effect level (NOAEL) of the flower was 8 g /(kg·BW), and no teratogenic effect was observed. Conclusion The flower of Citrus grandis var. Longanyou is actually non-toxic. Acute toxicity, genetic toxicity, teratogenicity and sub-chronic toxicity are negative under the condition of these experiments.