月柿提取物体外抗氧化活性研究

目的以月柿提取物为原料,研究其对2,2-联苯基-1-苦基肼基(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基的清除能力,并测定其多酚类物质没食子酸的含量。方法检测波长为518 nm,反应时间为40 min条件下,以IC_(50)值(清除率为50%时,抗氧化剂的浓度值)作为抗氧化能力评价指标,并采用高效液相色谱法测定其没食子酸含量。结果月柿提取物对于DPPH自由基有良好的清除作用,其IC50值为0.1868 mg/mL,与常用抗氧化剂作用相当,且月柿提取物中没食子酸含量为1.65 g/kg。结论月柿提取物具有很强抗氧化活性,作为化妆品及功能性食品原料有广阔的开发前景。

Study on the antioxidant activity of the extract from persimmon

Objective To investigate the scavenging ability of 2,2-biphenyl-1-pictylhydrazine (DPPH) free radicals by using extract of persimmon, and determine its polyphenols content--gallic acid. Methods Under the conditions of detection wavelength of 518 nm and reaction time of 40 mins, the IC50 value (antioxidant concentration value at 50% clearance rate) was used as the evaluation index of antioxidant capacity. And high performance liquid chromatography (HPLC) measurements were conducted to evaluate gallic acid content. Results The persimmon extract had a good scavenging effect on DPPH free radicals, and its IC50 value was 0.1868 mg/mL, which was equivalent to the common antioxidants.The gallic acid content in persimmon extract was 1.65 g/kg. Conclusion The extract of persimmon has strong antioxidant activity, and has broad development prospects as a raw material for cosmetics and functional foods.