食品和水中大肠埃希氏菌验证方法的比较

目的探讨IMViC检测实验培养基在大肠菌群验证实验中的适用性,优选相对快速确证为大肠菌群的培养基。方法比较IMViC检测实验培养基与国标法中验证实验所用培养基测定大肠埃希氏菌以及食品、生活饮用水、天然饮用矿泉水中大肠菌群不同时段的结果,对比所有样本确证为大肠菌群所需时间。结果测定大肠埃希氏菌以及食品、生活饮用水、天然饮用矿泉水中大肠菌群时, IMViC检测实验培养基6 h测定结果与国标法最终结果均无显著性差异(P0.05)。结论 IMViC检测实验培养基适用于大肠菌群验证试验,可根据实验室检测需求选择适用于大肠菌群快速检测实验的培养基。

Comparison of the methods for the confirmation of Escherichia coli in food and water

Objective To explore the applicability of IMViC test medium in the experiment of coliform group confirmation, and to select the medium that can confirm coliform group relatively quickly. Methods The results of different periods of time of coliform group in IMViC test medium and national standard method were compared, and the time needed for all samples to confirm coliform group was compared. Results There was no significant difference (P>0.05) between the results of IMViC test medium for 6 hours and the final results of national standard method in the determination of Escherichia coli, food, drinking water and natural mineral water. Conclusion IMViC test medium is suitable for coliform group verification experiment, and the medium suitable for coliform group rapid verification experiment can be selected according to the needs of laboratory test.