马铃薯青枯病菌日常快速检验方法的对比

目的对马铃薯青枯病菌日常快速检验的方法的对比。方法以日常监管抽检的出口马铃薯为材料,采用双抗夹心酶联免疫吸附法(double antibody sandwich enzyme linked immunosorbent assay,DAS-ELISA)以及分子生物学方法的实时荧光PCR(PT-PCR)法进行快速检测。结果通过连续多批次马铃薯检测的结果进行统计分析,试验结果显示,应用上述两种方法对马铃薯青枯病菌携带情况具有较高的灵敏度,特别是对处于潜伏期感染而没有表现出症状的马铃薯块茎的检测更为有效。结论 DAS-ELISA以及分子生物学方法的RT-PCR法对于马铃薯出口的快速检验快速通关具有重要的意义。

Comparison of routinequick tests for solanacearum in potato

This thesis with daily supervision sampling potato as the material, adopt the serology agglutination DAS ELISA and molecular biology method of real-time fluorescent PCR (rt-pcr) method for quick detection, through continuous multiple statistical analysis of the testing results of the batch of potato test results show that the application of the above two methods of potato solanacearum carrying case has high sensitivity, especially for in latent infection without showing symptoms of potato tuber detection is more effective, for potato solanacearum epidemic prevention and control is of great importance.