大孔树脂法纯化玉米须中槲皮素的工艺优化

目的获得大孔树脂法纯化玉米须中槲皮素的最优工艺条件。方法以吸附率、吸附量、洗脱率为指标,通过静态吸附与洗脱试验,确定综合指标最优树脂。通过单因素试验研究树脂质量、洗脱剂体积、洗脱剂体积分数对玉米须中槲皮素纯化效果的影响。最后利用L_(16)(4~5)正交表,以回收率为指标,对上述3个因素进行优化。结果通过筛选评价,D101型大孔树脂的综合性能最优。正交试验确定的最佳纯化工艺参数为:树脂质量1.5 g,洗脱剂体积15 m L,洗脱剂体积分数70%。在此条件下,通过3次验证试验,测得槲皮素的回收率为(91.41±1.63)%,纯化后样液中槲皮素纯度达到53.89%,较纯化前的纯度提高2.39倍。结论采用正交法优化大孔树脂法纯化玉米须中槲皮素的工艺具有可行性。

Optimization of purification process of quercetin in corn silk by macro-porous resin adsorption

Objective To investigate the optimum purification technical conditions of quercetin in corn silk by macro-porous resin adsorption. Methods Static adsorption and desorption experiments with indexes of the ratios of adsorption and desorption were explored to select the optimum resin. On the basis of single-factor tests, the recovery rate of quercetin was investigated by orthogonal experiments of L16 (45) with response to 3 main variables, including resin mass, eluent volume and eluent volume fraction. Results The D101 resin had the best purification effect. The optimum technical conditions were determined by orthogonal experiments as the resin mass was 1.5 g, eluent volume was 15 mL and eluent volume fraction was 70%. Under these conditions, the recovery rate of quercetin was (91.41±1.63)% by 3 times validation experiment. The purity of quercetin reached 53.89%, which was increased by 2.39 times compared with before purification. Conclusions It is feasible to use orthogonal method to get the optimum purification technical conditions of quercetin in corn silk by macro-porous resin adsorption.