六堡茶水提物对小鼠腹泻及肠道菌群的影响

目的 探讨六堡茶水提物对番泻叶诱导的小鼠腹泻及肠道菌群的影响。方法 采用六堡茶水提物对小鼠进行20 d的腹泻预防灌胃，从第16 d开始同时给予小鼠番泻叶煎剂诱导小鼠腹泻模型；试验结束时观察各组小鼠行为状态、体重、腹泻率及腹泻指数，检测盲肠内容物pH值及氨态氮含量、粪便肠道菌群数量，分析空肠绒毛形态结构。结果 与正常组相比，模型组小鼠体重显著减轻(P<0.05)、稀便率和腹泻指数均极显著上升(P<0.01)，说明番泻叶诱导小鼠腹泻模型构建成功。与模型组相比，六堡茶防治组特别是高剂量组能明显降低了稀便率和腹泻指数(P<0.05)，降低了小鼠盲肠内容物pH值及氨态氮含量(P<0.05)，增加了小鼠肠道双歧杆菌数量并减少肠球菌数量(P<0.05)，提升了小鼠空肠绒毛长度与隐窝深度的比值(V/C值, P<0.05)。结论 六堡茶对小鼠腹泻具有一定防治作用，其止泻作用可能与改善肠道内环境及修复双歧杆菌和肠球菌数量有关。

Effects of Liupao tea water extract on diarrhea and intestinal flora of mice

Objective To explore the influence of Liupao tea water extract on diarrhea and intestinal flora of mice induced by senna leaf. Methods Mice were administrated with Liupao tea water extract for 20 days for preventing diarrhea, and were simultaneously given senna leaf decoction to induce diarrhea from the 16th day. At the end of the experiment, the behavioral status, body weight, loose stools rate and diarrhea index of mice in each group were observed; the pH value and ammonia nitrogen level of cecum contents, and the number of fecal intestinal flora were detected, and the morphological structure of jejunum villi was analyzed. Results Compared with normal group, the weight loss significantly (P<0.05), the loose stools rate and diarrhea index of mice in model group were significantly increased simultaneously (P<0.01), indicated that the construction of diarrhea model induced by senna leaf was successful. Compared with the model group, the Liupao tea-treated group, especially the high-dose group, could significantly improve the loose stools rate and diarrhea indexof mice (P<0.05), reduce the pH value and ammonia nitrogen level of the cecum contents of mice(P<0.05), increase the number of Bifidobacterium, reduce the number of Enterococcus, and repair the villus height to crypt depth (V/C) in jejunum. Conclusion Liupao tea has a certain prevention effect on diarrhea in mice, and its anti-diarrhoea effect may be related to the improvement of intestinal environment and the reversal of content of the Bifidobacterium and Enterococcus.