6种农药多残留快速检测芯片的研制

目的 研制6种农药多残留快速检测芯片试纸条。方法 以硝酸纤维素膜为载体,农药抗原为捕获探针,时间分辨荧光微球为标记材料,通过对荧光微球粒径、硝酸纤维素膜、样品垫等反应条件进行优化,制作了一种包含6种抗原抗体组合的免疫芯片。结果 该免疫芯片可同时检测甲氰菊酯、多菌灵、克百威、吡虫啉、辛硫磷、灭蝇胺6种农药。本方法对6种农药的检测限分别为1 mg/kg、2 mg/kg、0.02 mg/kg、1 mg/kg、10 mg/kg、0.5 mg/kg；假阴性率、假阳性率均为0,准确度较高。结论 本研究为农药多残留快速检测分析提供了技术支撑,具有良好的实用价值。

Development of immunochip for multi-residue rapid detection of 6 kinds of common pesticides

Objective To develop an immunochip for multi-residue rapid detection of 6 kinds of common pesticides. Methods With nitrocellulose membrane as solid phase, pesticide antigen as capture probe, time-resolved fluorescent microsphere as labeling material, an immunochip containing 6 combinations of coating antigens and antibodies against corresponding pesticides was developed, by optimizing the reaction conditions of fluorescent microsphere particle size, nitrocellulose membrane and sample pad. Results This immunochip could be used for simultaneous detection of 6 kinds of common pesticides, including fenpropathrin, carbendazim, carbofuran, imidacloprid, phoxim and cyromazine. The detection limits were 1, 2, 0.02, 1, 10 and 0.5 mg/kg, respectively. The false negative rate and false positive rate were both 0, with high accuracy. Conclusion This immunochip assay provides technical support for the rapid detection of pesticide multi-residue, and has practical significance for guaranteeing the quality and safety of agricultural products.