| 表面等离子共振技术快速检测食品中的 玉米赤霉烯酮毒素 |
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| 引用本文： | 赵芳,涂晓波,黄欣迪,张恒,洪小柳,刘慧玲,吕敬章.表面等离子共振技术快速检测食品中的 玉米赤霉烯酮毒素[J].食品安全质量检测技术,2016,7(12):4849-4852. |
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| 作者姓名： | 赵芳 涂晓波 黄欣迪 张恒 洪小柳 刘慧玲 吕敬章 |
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| 作者单位： | 深圳出入境检验检疫局食品检验检疫技术中心,深圳出入境检验检疫局食品检验检疫技术中心,深圳出入境检验检疫局食品检验检疫技术中心,深圳出入境检验检疫局食品检验检疫技术中心,深圳出入境检验检疫局食品检验检疫技术中心,深圳出入境检验检疫局食品检验检疫技术中心,深圳出入境检验检疫局食品检验检疫技术中心 |
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| 摘 要： | 目的建立基于表面等离子共振(surface plasmon resonance,SPR)技术快速检测食品中玉米赤霉烯酮毒素(zearalenone,ZEN)的方法。方法采用表面自组装技术(self-assembled monolayer,SAM)在金膜的表面修饰羧基基团,将ZEN抗原与牛血清白蛋白(albumin from bovine serum,BSA)偶联物(ZEN-BSA)通过共价键固定在芯片的表面,采用竞争法检测样品中的玉米赤霉烯酮毒素。结果该方法的检测限为8.2 ng/m L,ZEN单克隆抗体与呕吐毒素、黄曲霉毒素B_1、赭曲霉毒素、伏马毒素等没有交叉反应,与α-玉米赤霉烯醇和β-玉米赤霉烯醇交叉反应率分别为15.3%和11.5%。结论本方法具有简便、快速和高灵敏度等优势,在食品中真菌毒素的快速检测方面具有潜在的应用价值。
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| 关 键 词： | 玉米赤霉烯酮 表面等离子共振 酶联免疫法 真菌毒素 |
| 收稿时间： | 2016/10/11 0:00:00 |
| 修稿时间： | 2016/12/8 0:00:00 |
Rapid detection of zearalenone in food by surface plasmon resonance technology |
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| ZHAO Fang,TU Xiao-Bo,HUANG Xin-Di,Zhang Heng,HONG Xiao-Liu,LIU Hui-Ling and LV Jing-Zhang.Rapid detection of zearalenone in food by surface plasmon resonance technology[J].Food Safety and Quality Detection Technology,2016,7(12):4849-4852. |
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| Authors: | ZHAO Fang TU Xiao-Bo HUANG Xin-Di Zhang Heng HONG Xiao-Liu LIU Hui-Ling and LV Jing-Zhang |
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| Affiliation: | Shenzhen Key Laboratory of Detection Technology R&D on Food Safety,Food Inspection and Quarantine Center,Shenzhen Entry-Exit Inspection and Quarantine Bureau,Shenzhen Key Laboratory of Detection Technology R D on Food Safety,Food Inspection and Quarantine Center,Shenzhen Entry-Exit Inspection and Quarantine Bureau,Shenzhen Key Laboratory of Detection Technology R D on Food Safety,Food Inspection and Quarantine Center,Shenzhen Entry-Exit Inspection and Quarantine Bureau,Shenzhen Key Laboratory of Detection Technology R D on Food Safety,Food Inspection and Quarantine Center,Shenzhen Entry-Exit Inspection and Quarantine Bureau,Shenzhen Key Laboratory of Detection Technology R D on Food Safety,Food Inspection and Quarantine Center,Shenzhen Entry-Exit Inspection and Quarantine Bureau,Shenzhen Key Laboratory of Detection Technology R D on Food Safety,Food Inspection and Quarantine Center,Shenzhen Entry-Exit Inspection and Quarantine Bureau,Shenzhen Key Laboratory of Detection Technology R D on Food Safety,Food Inspection and Quarantine Center,Shenzhen Entry-Exit Inspection and Quarantine Bureau |
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| Abstract: | |
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| Keywords: | zearalenone surface plasmon resonance enzyme linked immunosorbent assay mycotoxins |
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