高压液相色谱法检测饮料中维生素 B6

目的 建立饮料中维生素 B6 含量的高压液相色谱检测方法。方法 饮料样品经稀释、过滤后, 用高 压液相色谱仪进行测定, 测定结果与 GB/T 5009.154-2003《食品中维生素 B6 的测定》比较。结果 浓度范围 内线性关系良好, 相关系数 R 均在 0.999 以上。本方法的检出限(S/N≥3)为吡哆醇 15 μg/kg、吡哆醛 13 μg/kg、 吡哆胺 16 μg/kg。平均回收率为 98.9%~99.5%, 相对标准偏差为 0.88%~1.05%。使用高压液相色谱法测定饮料 中维生素 B6 含量与使用微生物方法检测得到的数据一致, 而高压液相色谱法所用时间比微生物法要短得多。 结论 高压液相色谱法测定饮料中维生素 B6 灵敏度高, 值得在实验室的日常检测中推广。

Detection of vitamin B6 in beverage by high pressure liquid chromatography

Objective To establish a rapid and effective method for the detection of vitamin B6 in beve- rage by high pressure liquid chromatography. Methods After dilution and filtration, samples was measured by high pressure liquid chromatography (HPLC) with fluorescence detector, and the data was compared with that obtained by the national standard microbial method. Results The data of vitamin B6 content in beverage detected by HPLC was consistent with that obtained by microbial method. But the detection time of HPLC was much shorter than that of the microbial method. Moreover, the operation of HPLC was simple, and the repeata- bility and accuracy of data were much higher as well. Conclusion HPLC is a sensitive method for the detec- tion of vitamin B6 in beverage, and it could be applied to the daily detection in laboratory.