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菌落总数质控样的制备及其在能力验证中的应用
引用本文:陈怡文,张晓东,宋佳兴,刘雅丹,赵 萌,余 文,任 秀,崔生辉.菌落总数质控样的制备及其在能力验证中的应用[J].食品安全质量检测技术,2019,10(12):3772-3776.
作者姓名:陈怡文  张晓东  宋佳兴  刘雅丹  赵 萌  余 文  任 秀  崔生辉
作者单位:中国食品药品检定研究院;北京三药科技开发公司
基金项目:科技部“食品安全关键技术研发”重点专项项目(2017YFC1601400)
摘    要:目的制备均匀性和稳定性符合能力验证要求的质控样品,并将质控样品用于菌落总数的能力验证。方法本次考核样品由高浓度(3×10~4 CFU)、低浓度(1×10~4 CFU)2个样品组成,随机抽取质控样品进行稳定性及均匀性的检验,对其进行评价。本设计通过对实验室进行随机分组来发放样品,防止能力验证活动中各实验室进行串通。结果 65家实验室参加本次考核活动,整体满意率为80%,本次能力验证可以真实地反映参试单位的检测水平。结论本次菌落总数能力验证样品可用于质控样品使用。

关 键 词:菌落总数    能力验证    质控样品
收稿时间:2019/3/8 0:00:00
修稿时间:2019/6/3 0:00:00

Preparation of the quality control samples for aerobic plate count and the application in the proficiency test
CHEN Yi-Wen,ZHANG Xiao-Dong,SONG Jia-Xing,LIU Ya-Dan,ZHAO Meng,YU Wen,REN Xiu and CUI Sheng-Hui.Preparation of the quality control samples for aerobic plate count and the application in the proficiency test[J].Food Safety and Quality Detection Technology,2019,10(12):3772-3776.
Authors:CHEN Yi-Wen  ZHANG Xiao-Dong  SONG Jia-Xing  LIU Ya-Dan  ZHAO Meng  YU Wen  REN Xiu and CUI Sheng-Hui
Affiliation:National institutes for food and drug control,National Institutes for Food and Drug Control,Beijing SanYao Science & Technology Development Co.,,National Institutes for Food and Drug Control,National Institutes for Food and Drug Control,National Institutes for Food and Drug Control,National Institutes for Food and Drug Control and National Institutes for Food and Drug Control
Abstract:Objective To prepare quality control samples with uniformity and stability those meet the requirements for proficiency testing, and verify the ability of the quality control samples for the total number of colonies. Methods The test samples were composed of 2 samples with high concentration (3×104 CFU) and low concentration (1×104 CFU). Randomly selected samples were tested the stability and uniformity. This design distributed samples by randomly grouping laboratories to prevent collusion in laboratories in proficiency testing activities. Results Totally 65 laboratories participated in this proficiency test, 80% of the laboratories with excellent results. This proficiency test could truly reflect the detection level of the participating units. Conclusion This verification samples of total number of colony can be used for quality control samples.
Keywords:aerobic plate count  proficiency test  quality control samples
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