高效液相色谱法测定话梅中阿力甜的不确定度评定

目的评定高效液相色谱法测定话梅中阿力甜的不确定度。方法根据GB5009.263-2016《食品安全国家标准食品中阿斯巴甜和阿力甜的测定》测定话梅中的阿力甜含量,按照JJF 1059.1-2012《测量不确定度评定与表示》、CNAS-GL 05:2011《测量不确定度要求的实施指南》及CNAS-GL 06:2018《化学分析中不确定度的评估指南》,建立相关测量模型,对不确定度来源进行分析,对分析得出的不确定度分量进行逐个量化,最终进行测定结果的不确定度评定。结果在99%置信区间下,k=2.576,话梅中阿力甜的含量为(0.0823±0.0059)g/kg。结论阿力甜标准溶液配制引入的不确定度和最小二乘法拟合标准曲线求试样含量引入的不确定度是最主要的不确定度来源。

Uncertainty evaluation of determination of alitame in preserved plum by high performance liquid chromatography

Objective To evaluate the uncertainty of determination of alitame in preserved plum by high performance liquid chromatography. Methods Alitame in preserved plum was determined according to GB 5009.263-2016 National food safety standard-Determination of aspartame and alitame in food. The relevant measurement model was established according to JJF 1059.1-2012 Evaluation and expression of uncertainty in measurement, CNAS-GL 05:2011 Guidance on the application of the requirements for measurement uncertainty and CNAS-GL 06:2018 Guidance on evaluating the uncertainty in chemical analysis. The sources of uncertainty were analyzed, the components of uncertainty were quantified, and the uncertainty of measurement results was evaluated finally. Results In the 99% confidence interval, k=2.576, the content of alitame in preserved plum was (0.0823± 0.0059) g/kg. Conclusion The main sources of uncertainty are the uncertainty introduced in the preparation of alitame standard solution and the determination of sample content by fitting standard curve with least square method.