齿瓣石斛提取物对SD大鼠的致畸作用研究

目的研究齿瓣石斛对SD大鼠的胚胎毒性与致畸毒性。方法孕SD鼠随机分为4组,分别为齿瓣石斛4.5(低剂量)、9.0(中剂量)、18.0(高剂量)g/kg剂量组和阴性对照组,每组大于17只。在受孕的第7～16 d,每天经口给予受试物,实验期间记录孕鼠的一般状况、体重,于受孕第20 d剖检孕鼠,检查孕鼠的体重及妊娠情况,胎鼠的体重、身长、尾长与外观、骨骼、内脏的畸形情况。结果各剂量组孕鼠增重、活胎率、吸收胎率、活胎体重、身长及尾长与阴性对照组比较,差异均无统计学意义(P0.05)。各剂量组和阴性对照组胎鼠的外观、骨骼及内脏未见畸形。结论在本实验条件下,未发现齿瓣石斛对SD大鼠的胚胎毒性和致畸毒性。

Teratogenic effects of Dendrobium devonianum Paxton extract on SD rats

Objective To investigate the embryo toxicity and teratogenicity of Dendrobium devonianum Paxton in SD rats. Methods SD rats successfully impregnated were divided into 4 groups (more than 17 rats per group), including three dosage groups (4.5, 9.0, 18.0 g/kg) and one negative control group. During gestation from 7-16 d, animals were orally gavaged with caulis dendrobii devoniani once daily, clinical manifestations and weight of the pregnant rats were collected during the study. On the 20th day pregnant rats were euthanized and dissected, and the body length, tail length, body weight of the fetus and length of fetus and the appearance, visceral and skeletal malformations of fetus were examined. Results No statistically significant differences were found in all the assessed indices (including the weight gain of pregnant rats, average live fetus, absorptive fetus ratio, body length, tail length, and body weight of the fetus) between dosage and control groups (P>0.05). Conclusion Under the experimental conditions, caulis dendrobii devoniani shows no significant effects on the toxicity of SD pregnant rats, which has no apparent teratogenicity and embryotoxicity.