超高效液相色谱-串联质谱法同时检测糕点中6种甜味剂

目的建立超高效液相色谱-串联质谱法同时测定糕点中6种常用合成甜味剂的分析方法。方法选用超纯水作为提取溶剂,涡旋和超声提取后,低温离心,取部分上清液加入正己烷除脂,Waters Atlantis■T3色谱柱、甲醇-5 mmol/L甲酸铵(含0.1%甲酸)作为流动相、亲水亲脂平衡型固相萃取柱HLB(hydrophile-lipophile balance)净化。结果6种甜味剂在质量浓度为10~200 ng/mL的曲线范围内呈良好线性关系,相关系数r均大于0.999,平均加标回收率在85.0%-98.2%之间,相对平均偏差(relative standard deviation,RSD)为1.3%~6.7%。结论该方法具有前处理简单、灵敏度高、检测速度快等优点,适合糖精钠、甜蜜素、三氯蔗糖、阿斯巴甜、阿力甜、纽甜的检测,但不适用于安赛蜜的检测。

Simultaneous determination of 6 kinds of sweeteners in pastries by ultra performance liquid chromatography-tandem mass spectrometry

Objective To establish a method for simultaneous detection of 6 commonly used synthetic sweeteners in pastries by ultra performance liquid chromatography-tandem mass spectrometry.Methods Using ultrapure water as the extraction solvent,after vortexing and ultrasonic extraction,cryogenic centrifugation,took part of the supernatant and added n-hexane for degreasing,Waters Atlantis■T3,methanol-0.1%formic acid with 5 mmol/L ammonium formate were purified as mobile phase and hydrophile-lipophile balance equilibrium solid phase extraction column purification.Results The 6 sweeteners had good linear relationships within the curve ranges of the concentration of 10-200 ng/mL,the correlation coefficients were greater than 0.999,the average values of the addition were in the range from 85.0%-98.2%with the relative standard deviation(RSDs)were 1.3%-6.7%.Conclusion This method has the advantages of simple pretreatment,good purifying effect,high sensitivity and fast detection rate,and it is suitable for the detection of saccharin sodium,sodium cyclamate,sucralose,aspartame,alitame and neotame,but not suitable for the detection of acesulfame K.