气相色谱仪柱温箱升温程序的调试方法

目的探究调试气相色谱仪柱温箱升温程序方法。方法比较分析柱温箱升温程序调试过程中柱流速、升温速率、不同温度下的保持时间等调试步骤对分离色谱峰的影响,确定调试步骤的前后顺序及调试方法。结果调试柱温箱升温程序后,在丁体六六六和氧化氯丹中间的干扰峰,出峰于丁体六六六和氧化氯丹后面,完全分离出来,减少了干扰峰对结果的影响。结论该方法可以更快速、有效的调试柱温箱升温程序,分离干扰峰,适合气相色谱仪柱温箱升温程序的调试。

Debugging method of temperature rising program in column incubator of gas chromatography

Objective To explore the method of debugging the heating procedure of gas chromatograph column incubator. Methods In the process of debugging the heating program of the column incubator, the effects of debugging steps such as column velocity, heating rate and holding time at different temperatures on separating chromatographic peaks were compared and analyzed, and the sequence of debugging steps and debugging methods were determined. Results After adjusting the temperature rising program of column temperature box, the interference peak between γ-BHC and chlordane oxide was separated completely, which reduced the influence of interference peak on the results. Conclusion This method can debug the temperature rising program of column incubator more quickly and effectively, and separate the interference peak, which is suitable for the adjustment of temperature rising program of column incubator of gas chromatograph.