测试片法在食品菌落总数检测中的应用研究

目的探究菌落总数测试片法检测食品中菌落总数的可行性。方法用菌落总数测试片法和国家标准方法同时对制备的菌悬液、自然污染食品样品和人工污染食品样品的菌落总数进行检测,采用t-检验分析2种方法检测结果的差异,采用Pearson相关分析确定2种检测方法之间的相关性。结果菌悬液和自然污染食品样品菌落总数测试片法的变异系数为0.16%~3.76%,人工污染食品样品的变异系数为0.78%~8.61%;菌落总数测试片与国家标准方法在不同类型食品样品检测结果之间没有显著性差异(P<0.05),2种方法的检测结果呈正相关(r2>0.976,P<0.001)。结论菌落总数测试片法的检测效果与国家标准方法相当,可作为替代方法,用于检测食品中的菌落总数。

Application of the test tablet method in the detection of total number of food colonies

Objective To analyze the feasibility in detection of aerobic plate count in food by applying paper plate method. Methods The total number of colonies of the prepared bacterial suspensions, natural contaminated food samples and artificial contaminated food samples were tested by paper plate method and national standard method at the same time. The difference between two detection methods were analyzed by t-test. A Pearson correlation analysis method were also applied to evaluate the correlation of the two methods. Results The coefficient of variation of bacterial suspension and total bacterial colony of naturally contaminated food samples were 0.16%-3.76%, the coefficient of variation of artificially contaminated food samples were 0.78%-8.61%. Difference in aerobic plate count between two detection methods was not significant(P<0.05). There was a positively correlation in results of two detection methods(r~2>0.976, P<0.001). Conclusions The detection effect of the paper plate method is equivalent to the national standard method, which can be applied as an alternative method to detect aerobic plate count in food.