首页 | 本学科首页   官方微博 | 高级检索  
     

免疫磁珠的制备及其在食品安全检测中的应用
引用本文:柳心梅,田巍,肖治理.免疫磁珠的制备及其在食品安全检测中的应用[J].食品安全质量检测技术,2018,9(18):4775-4780.
作者姓名:柳心梅  田巍  肖治理
作者单位:四川卫生康复职业学院,四川卫生康复职业学院,四川卫生康复职业学院,四川卫生康复职业学院,四川卫生康复职业学院
基金项目:四川省教育厅科研计划项目(16ZB0531)
摘    要:目的 研究胃肠液对纳豆激酶纤溶活性的影响。方法 模拟人体胃液、肠液中的生理过程, 采用纤维蛋白平板法, 对粗酶液和含豆固体粉碎样品2种形态的纳豆激酶进行纤溶活性研究。结果 粗酶液和含豆固体粉碎样品在人工胃液处理5 h左右时, 活性分别下降到对照组的28%和35%, 说明2种状态的酶在酸性的胃液中都不稳定, 大部分活性丧失; 粗酶液和含豆固体粉碎样品在人工胃液处理4 h后, 再用人工肠液处理 5 h, 活性分别下降到对照组的89%和91%; 说明在人工肠液中2种状态的酶都较稳定, 都保持较高的酶活性。结论 为避免胃酸的破坏作用, 建议将纳豆激酶制成肠溶性的产品服用。

关 键 词:人工胃液    人工肠液    纳豆激酶    活性研究    溶栓药物
收稿时间:2018/5/10 0:00:00
修稿时间:2018/9/5 0:00:00

Preparation of immunomagnetic beads and their application in food safety detection
LIU Xin-Mei,TIAN Wei and XIAO Zhi-Li.Preparation of immunomagnetic beads and their application in food safety detection[J].Food Safety and Quality Detection Technology,2018,9(18):4775-4780.
Authors:LIU Xin-Mei  TIAN Wei and XIAO Zhi-Li
Affiliation:Sichuan health rehabilitation Career Academy,Sichuan health rehabilitation Career Academy,Sichuan health rehabilitation Career Academy,Sichuan health rehabilitation Career Academy and Sichuan health rehabilitation Career Academy
Abstract:Objective To study the effect of gastrointestinal fluid on the fibrinolytic activity of nattokinase. Methods The physiological processes in the human gastric and intestinal juice were simulated. The fibrinolytic activity of nattokinase in crude enzyme solution and solid crushing sample containing bean were studied by fibrinoid plate method. Results When the crude enzyme solution and solid crushing sample containing bean were treated with the artificial gastric juice for about 5 hours, the activity of nattokinase decreased to 28% and 35% of the control group respectively, indicating that the enzymes in the 2 kinds of state were unstable in the acidic gastric juice and most of the activity was lost. When the crude enzyme solution and solid crushing sample containing bean were treated with the artificial gastric juice for about 4 hours, and then the artificial intestinal solution was used for 5 hours, the activity decreased to 89% and 91% of the control group respectively, and the results showed that the enzymes in the two states were stable and had high enzyme activity. Conclusion It recommends to use nattokinase as an enteric soluble product in order to avoid the damage of gastric acid.
Keywords:artificial gastric juice  artificial intestinal juice  nattokinase  activity study  thrombolytic drugs
点击此处可从《食品安全质量检测技术》浏览原始摘要信息
点击此处可从《食品安全质量检测技术》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号