恒温荧光核酸检测与国标法在副溶血性弧菌检验能力验证中的应用与分析

目的通过参加中国食品药品检定研究院组织的副溶血性弧菌检验能力验证活动,提高实验室检测副溶血性弧菌的准确率。方法对能力验证标号为CODE1～CODE3的3组鱼蛋白粉样品,使用恒温荧光核酸技术与国标GB 4789.7-2013食品安全国家标准食品微生物学检验副溶血性弧菌检验进行副溶血性弧菌的检测。结果使用恒温荧光核酸检出CODE2为阳性结果,与GB4789.7-2013国家标准方法检出的阳性结果一致,证明CODE2为副溶血性弧菌。结论通过此次能力验证,使得实验室副溶血性弧菌的检测能力得到了有效提高,为快速检测副溶血性弧菌提供参考。

Application and analysis of thermostatic fluorescence nucleic acid detection and national standard method in the proficiency testing of Vibrio parahaemolyticus

Objective To improve the accuracy of laboratory detection of Vibrio parahaemolyticus by participating in the proficiency testing organized by National Institutes for Food and Drug Control. Methods Vibrio parahaemolyticus was detected by thermostatic fluorescence nucleic acid technology and national standard GB 4789.7-2013 National food safety standard-Food microbiological analysis-Vibrio parahaemolyticus test in 3 groups of fish protein powder samples with CODE1-CODE3 for proficiency testing. Results CODE2 was detected as a positive result by thermostatic fluorescence nucleic acid, which was consistent with the positive result detected by GB 4789.7-2013 national standard method, proving that CODE2 was Vibrio parahaemolyticus. Conclusion Through this proficiency testing, the detection ability of Vibrio parahaemolyticus in the laboratory has been effectively improved, providing a reference for rapid detection of Vibrio parahaemolyticus.