| 优化高效液相色谱-串联质谱法检测水产品中 孔雀石绿及隐色孔雀石绿的残留量 |
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| 引用本文： | 李凯华,张玲,闫清华,徐媛原,巫新南.优化高效液相色谱-串联质谱法检测水产品中 孔雀石绿及隐色孔雀石绿的残留量[J].食品安全质量检测技术,2018,9(6):1350-1357. |
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| 作者姓名： | 李凯华 张玲 闫清华 徐媛原 巫新南 |
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| 作者单位： | 深圳市农产品质量安全检验检测中心 |
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| 摘 要： | 目的对GB/T 19857-2005中检测水产品中孔雀石绿及隐色孔雀石绿残留量的高效液相色谱-串联质谱法(high performance liquid chromatography-tandem mass spectrometry,HPLC-MS/MS)进行优化。方法经匀质的水产品样品,加20 mL乙腈和5 g酸性氧化铝旋涡振荡,取4 mL提取液,35℃水浴氮吹近干,用7:3(V:V)乙酸铵和乙腈混合溶液1 mL定容,过0.22μm滤膜。样液用HPLC-MS/MS进行检测,以氘代孔雀石绿和氘代隐色孔雀石绿为内标物进行定量。结果本实验的线性范围在0.5~10.0μg/kg,相关系数为0.9994和0.9995,孔雀石绿和隐色孔雀石绿的平均检出限达到0.0241μg/kg和0.0475μg/kg,方法的回收率在91.1%~107.3%之间,相对标准偏差在1.0%~10.4%之间。结论该方法灵敏度高,准确性好,与GB/T 19857-2005相比,简化了前处理步骤,回收率稳定。通过验证,该方法适用于开展水产品中孔雀石绿及隐色孔雀石绿残留的检测。
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| 关 键 词： | 高效液相色谱-串联质谱法 孔雀石绿 隐色孔雀石绿 水产品 |
| 收稿时间： | 2018/2/24 0:00:00 |
| 修稿时间： | 2018/3/26 0:00:00 |
Optimization of methods for determination of malachite green and leucomalachite green in aquatic products by high performance liquid chromatography-tandem mass spectrometry |
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| LI Kai-Hu,ZHANG Ling,YAN Qing-Hu,XU Yuan-Yuan and WU Xin-Nan.Optimization of methods for determination of malachite green and leucomalachite green in aquatic products by high performance liquid chromatography-tandem mass spectrometry[J].Food Safety and Quality Detection Technology,2018,9(6):1350-1357. |
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| Authors: | LI Kai-Hu ZHANG Ling YAN Qing-Hu XU Yuan-Yuan and WU Xin-Nan |
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| Affiliation: | Agricultural Product Quality Safety Inspection and Testing Center of Shenzhen,Agricultural Product Quality Safety Inspection and Testing Center of Shenzhen,Agricultural Product Quality Safety Inspection and Testing Center of Shenzhen,Agricultural Product Quality Safety Inspection and Testing Center of Shenzhen and Agricultural Product Quality Safety Inspection and Testing Center of Shenzhen |
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| Abstract: | |
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| Keywords: | high performance liquid chromatography-tandem mass spectrometry malachite green leucomalachite green aquatic products |
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