HPLC测定安神颗粒中酸枣仁皂苷A及B的含量

目的建立测定安神颗粒中酸枣仁皂苷A及B含量的方法，提供该制剂质量控制标准。方法应用高效液相色谱法．蒸发光散射检测器（HPLC—ELSD），Kromasil C18色谱柱（4．6mm×200mm，5μm），流动相：甲醇-水-冰醋酸（65：35：1），流速：1．0mL／min，柱温25℃，氮气流速2．0L／min，漂移管温度94℃，测定安神颗粒中酸枣仁皂苷A和B含量。结果酸枣仁皂苷A及B平均回收率分别为100．2％（RSD=1．21％）和99．88％（RSD=1．32％）。结论此方法操作简便，结果准确、可靠，可有效控制制剂的质量。

Determination of Jujuboside A and B in Anshen Granules by HPLC

Objective To establish a method for the determination ofjujuboside A and B in Anshen Granules and to provide the quality control standard for preparation. Methods Jujuboside A and B in Anshen Granules were determined by high performance liquid chromatography-evaporative light-scattering detector（HPLC-ELSD）. The column was Kromasil C18 （4.6 min× 200 mm,5μm） and the mobile phase consisted of methanol-water-glacial acetic acid （65：35：1） with the flow rate of 1.0 mL/min. The column temperature was 25 ℃ and the nitrogen flow rate was 2.0 L/min and the temperature of the drift tube was 94℃. Results The average recovery ofjujuboside A and B were 100.2 % （RSD 1.21%）和99.88 % （RSD 1.32 %） , respectively. Conclusion The method is simple, accurate and reliable for the quality control of Anshen Granules.