响应面法优化猪脾免疫活性多肽提取工艺研究

目的通过响应面法，优化猪脾中免疫活性多肽的提取工艺。方法选择适当的酶水解猪脾脏，并在单因素试验的基础上，采用Box—Behnken设计优化提取工艺，建立各因素与提取液中多肽含量的数学模型，最后采用淋巴细胞转化试验初步验证其活性。结果获得了猪脾中免疫活性多肽提取工艺：料液比1：2．6g／mL，温度40℃，pH7．0，胰蛋白酶浓度485U／g，中性蛋白酶浓度1711U／g，每100g含活性多肽2．930g，与理论计算值2．929g基本一致，活性约为对照组的1．5倍。结论确定了猪脾免疫活性多肽最佳提取工艺。

Pig Spleen Immunity Activity Peptides Extraction Process Optimization by Response Surface Method

Objective To optimize pig spleen immunity activity peptides extraction process by response surface method. Methods Choose appropriate enzymes for hydrolysis of pig spleen, using the design of Box-Behnken to optimize extraction process on the basis of single factor test, and establish a mathematical model of all factors for the content of peptides. Finally, a lymphocyte transformation test was conducted for the preliminary validation of its activity. Results The best extraction process was the proportion of material and liquid 1：2.6 g/mL, temperature： 40 ℃, pH 7.0, concentration of trypsin 485 U/g, concentration of neutral protease 1711 U/g. The content of active peptides was 2.930 g/100g, basically the same level of theoretical value 2.929g/100g. Activity was about 0.5 times higher than the control group. Conclusion The optimum extraction process of pig spleen immunity activity peptides was determined as the proportion of material and liquid 1：2.6 g/mL, temperature 40 ℃, pH 7.0, concentration of trypsin 485 U/g, concentration of neutral protease 1711 U/g.