Elemental Content and Total Antioxidant Activity of <Emphasis Type="Italic">Salvia fruticosa</Emphasis> |
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Authors: | Ioannis N Pasias Eleni G Farmaki Nikolaos S Τhomaidis Efrosini A Piperaki |
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Affiliation: | (1) Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Panepistimiopolis Zografou, 15771 Athens, Greece; |
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Abstract: | The determination of 18 elements (V, Cr, Cu, Co, Se, Sr, Sn, Sb, Ba, Bi, Pb, Cd, As, Ni, Mn, Fe, Mg and Zn) in leaves, flowers
and the infusion from Salvia fruticosa, a sage grown in Greece, is described. For this purpose, flame atomic absorption spectrometry has been used for the determination
of Fe, Mg, Zn and inductively coupled plasma-mass spectrometry has been used for the determination of V, Cr, Cu, Co, Se, Sr,
Sn, Sb, Ba, Bi, Pb, Cd, As, Ni, Mn using 45Sc 72Ge, 115In and 232Th as internal standards. The elemental content was found to be in the range of 0.01 (Bi)-30.8 (Mn) mg/Kg (leaves), 0.30 (Bi)-39.1
(Mn) mg/Kg (flowers), 0.003 (Sb)-20.4 (Mn) mg/Kg (infusion) for V, Cr, Cu, Co, Se, Sr, Sn, Sb, Ba, Bi, Pb, Cd, As, Ni, Mn
and in the range of 0.07 (Zn)-3.21 (Mg) g/kg (leaves) for Fe, Mg and Zn. The majority of the samples were collected from six
sites in the island Crete and transplanted and grown in a model farm. Chemometric techniques were used to investigate the
original site classification according to their elemental content, and it was proved that the initial cultivation sites were
characterized by only five elements (Sb, V, Zn, Cd and Cr). The application of factor analysis revealed significant correlation
between certain elements, denoting their common sources. In addition, the total antioxidant activity of the herbal preparation
was determined by measuring the 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity. Microwave-assisted extraction (MAE)
was used to extract total antioxidants and the effect of temperature, time and solvent in the extraction efficiency was investigated.
The determination of the antioxidant activity was based on the % inhibition of the absorbance signal of the radical DPPH at
515 nm, after the addition of herbal’s extract. The IC50 values were found to be in the range of 10.6–40.1 mg/L. |
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