Development and Validation of a Method for Determination of Residual Nitrite/Nitrate in Foodstuffs and Water After Zinc Reduction |
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Authors: | Leonardo Merino |
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Affiliation: | (1) Swedish National Food Administration, P.O. Box 622, 751 26 Uppsala, Sweden;(2) Swedish University of Agriculture Sciences, 750 07 Uppsala, Sweden |
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Abstract: | An environmentally friendly and cost-effective spectrophotometric method to analyze nitrate and/or nitrite was developed.
The method is based on reduction of nitrate with zinc powder (instead of the cadmium or enzymes used in the standard methods
approved by ISO/CEN). The initial nitrite concentration and total nitrite after reduction are determined by the very sensitive
and widely used diazotization-coupling Griess reaction. A single-laboratory validation was applied in five different matrices
(vegetable, meat product, baby food, dairy product, and surface water). The results show that the new method fulfills the
international criteria for precision and recovery. The limit of detection for several matrices, calculated using fortified
samples, ranged from 3 to 5 mg/kg for both nitrite and nitrate. Furthermore, the results obtained were in good agreement with
those obtained using the CEN method (HPLC) and the ISO method (Cd reduction). |
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Keywords: | Nitrate and Nitrite Analysis Environmentally Friendly Spectrophotometry Validation Analysis of Variance |
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