酶法催化酯交换反应制备脂肪酸甲酯工艺研究

利用凯泰中性脂肪酶催化菜籽油与甲醇酯交换制备脂肪酸甲酯,相比而言,采用经乳化后菜籽油作反应原料具有更高转化率;为提高转化率,采用响应面实验设计和分析方法对菜籽油酯交换反应条件进行优化,得到最佳工艺条件:反应温度47℃,反应时间34小时,醇油摩尔比为4.5∶1,脂肪酶用量420UI;在此工艺条件下,酯交换反应转化率达95.81%。采用GC–MS技术分析所得样品,结果表明,菜籽油脂肪酸甲酯主要组份为油酸甲酯。

Study on fatty acid methyl ester production by enzymatic transesterification reaction

The KaiTai lipase was used to catalyze transesterification reaction of rapeseed oil with methanol to prepare fatty acid methyl este(rFAME). Compared with rapeseed oil,the emulsified rapseseed oil has higher transformation ratio. In order to increase the transformation tatio,the response surface methodology was used to optimize the transesterification reaction conditions of rapeseed oil, and the optimized conditions of the reaction was found:the reaction temperature is 47℃,reaction time is 34 h,molar ratio of methanol and oil is 4.5∶1,and the dosage of lipase is 450UI. Under these optimal conditions,the transformation ration reached 95.81%. This sample was analyzed by GC–MS technique,the results showed that the main component of FAME from rapeseed oil is methyl oleate.