超声波辅助酶解黑豆蛋白工艺优化

利用不同蛋白酶酶解黑豆蛋白,根据水解度选择最佳用酶为碱性蛋白酶,采用超声波辅助酶法提取黑豆肽；分析超声波处理时间、功率、加酶量、pH、酶解时间及底物浓度对水解度及二苯代苦味酰基自由基( DPPH·)清除能力影响.在单因素实验基础上,依据响应面分析确定最优提取工艺条件为:超声功率1029.27 W、酶解pH 8.64、底...

Optimization of ultrasonic-assisted enzymatic hydrolyzing of black-soybean protein

Different proteases were used to hydrolyze black-soybean protein,according to the protein hydrolysis degree(DH).Alcalase protease was selected as the most appropriate enzyme.Ultrasound pretreatment was applied to the Alcalase protease hydrolysis of black-soybean protein.In the singlefactors analysis,the influences of the ultrasound time,power,substrate concentration,pH,temperature and enzyme concentration on the protein hydrolysis degree and DPPH ? scavenging activity were studied. The response surface analysis were used to optimize the condition of enzyme hydrolysis,the results showed that the optimum condition for preparing black-soybean peptides with high-DPPH ? scavenging activity were:ultrasound 20 min,ultrasound power 1029.27w,substrate concentration 4.04%,pH 8.64, temperature 50℃,enzyme concentration 4.10%.Under this condition,the DPPH? scavenging activity of the peptides obtained is 86.37%.