| 高效液相色谱法测定奶粉及其他乳制品中的L-羟脯氨酸 |
| |
| 引用本文： | 金苏英,林笑容,赵志红,朱慧,施文蓉.高效液相色谱法测定奶粉及其他乳制品中的L-羟脯氨酸[J].饮料工业,2009,12(7):28-30. |
| |
| 作者姓名： | 金苏英 林笑容 赵志红 朱慧 施文蓉 |
| |
| 作者单位： | 杭州娃哈哈集团有限公司研发中心,浙江杭州,310018 |
| |
| 摘 要： | 建立了柱前衍生反相高效液相色谱法测定奶粉及其他乳制品中L-羟脯氨酸的方法.采用酸水解样品,经6-氨基喹啉基-N-羟基琥珀酰亚氨基甲酸酯(AQC试剂)衍生水解样品中的L-羟脯氨酸,以磷酸盐缓冲溶液、乙腈、水梯度洗脱,流速1.0ml/min,通过symmetry C18柱将L-羟脯氨酸与其他18种氨基酸分离.紫外检测器在248nm波长下检测.测定结果显示,在0～2.05mmol/L范围内,标准曲线呈良好的线性关系(r=0.9991),加样回收率为95.1%～101%,方法精密度RsD(n=7)为1.3%.该方法具有简单、稳定、灵敏度高、准确等优点.
|
| 关 键 词： | 柱前衍生 反相高效液相色谱法 L-羟脯氨酸 AOC试剂 梯度洗脱 |
Determination of hydroxyproline in milk powder and other dairy products by HPLC |
| |
| JIN Su-ying,LIN Xiao-rong,ZHAO Zhi-hong,ZHU Hui,SHI Wen-rong.Determination of hydroxyproline in milk powder and other dairy products by HPLC[J].Beverage Industry,2009,12(7):28-30. |
| |
| Authors: | JIN Su-ying LIN Xiao-rong ZHAO Zhi-hong ZHU Hui SHI Wen-rong |
| |
| Affiliation: | R & D Department of Hangzhou Wahaha Group Co.;Ltd.;Hangzhou 310018;Zhejiang;China |
| |
| Abstract: | The method of determining hydroxyproline (Hyp) in milk powder and other dairy products was developed by pre-column derivation RP-HPLC. After acid hydrolysis,Hyp in samples was derived with AQC. A gradient elution was used to separate Hyp from other 18 amino acids by symmetry C18 column. And Hyp was determined with PDA detector at 248.0 nm. The mobile phase was phosphate buffered solution-water-acetonitrile. Good linearity was observed within the range from 0 to 2.05 mmol/L(r=0.99991). The recoveries were 95... |
| |
| Keywords: | |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
