蛋白酶水解绿茶籽粉的工艺优化

以脱脂绿茶籽粉为原料，用不同来源的蛋白酶在最适条件下对其进行水解，确定绿茶籽粉最适水解酶；并以水解度为指标，设计了4因素3水平试验，运用Design—Expert8．0．6统计分析软件，对酶解工艺进行优化。研究结果：绿茶籽粉蛋白酶解的最适酶为Alcalase；最佳水解工艺：pH8．85、酶用量938．6U／g、温度54．1℃、水解时间4．3h。在此条件下，预测水解度为71．2％，验证试验得到的水解度为71．66％，与预测值非常接近，证明研究中分析所得优化模型是可靠的。

Optimization of hydrolysis process of green tea seed powder by protease

Proteases of different origins were used for the hydrolysis of defatted green tea seed powder under the optimum conditions to determine the optimal hydrolase;with hydrolysis degree as the indicator,an L9(34) experiment was designed to optimize the hydrolysis process by Design-Expert 8.0.6.The results indicated that Alcalase was the optimal hydrolase;the optimum hydrolysis parameters included pH 8.85,enzyme dosage 938.6 U/g,54.1℃ and 4.3 h.Under these conditions,the hydrolysis degree obtained in the verification experiment was 71.66%,very close to the predicted value of 71.2%,which proved that the optimization model was feasible.