| 利用HPLC-MS/MS技术进行羟自由基检测的分析方法研究 |
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| 引用本文： | 杨 芬,张瑞萍,贺玖明,再帕尔&#,阿不力孜.利用HPLC-MS/MS技术进行羟自由基检测的分析方法研究[J].质谱学报,2006,27(Z1):59-60. |
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| 作者姓名： | 杨 芬 张瑞萍 贺玖明 再帕尔&# 阿不力孜 |
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| 作者单位： | 中国医学科学院，中国协和医科大学药物研究所,北京 100050 |
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| 摘 要： |
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Determination of Hydroxyl Radical by Liquid Chromatography Combined with Electrospray Ionization Tandom Mass Spectrometry |
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| YANG Fen,ZHANG Rui-ping,HE Jiu-ming,ZEPERAbliz.Determination of Hydroxyl Radical by Liquid Chromatography Combined with Electrospray Ionization Tandom Mass Spectrometry[J].Journal of Chinese Mass Spectrometry Society,2006,27(Z1):59-60. |
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| Authors: | YANG Fen ZHANG Rui-ping HE Jiu-ming ZEPERAbliz |
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| Affiliation: | Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
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| Abstract: | Hydroxyl radical (?OH) can cause severe damage to cells and tissues. However, its analysis is very difficult for its high reactivity and very short life. This paper present a HPLC-MS/MS method for indirectly detecting ?OH by determining DMPO-OH, its addut with spin trap DMPO ( 5,5-dimethyl-1-pyrroline N-oxide ), with the N-Methyl-2-Pyrrolidone (NMP) used as internal standard. The ratio of the peak area of the DMPO-OH and NMP negatively correlate with the concentration of ?OH. The results show that the method is simple, rapid, precise in both the determination of ?OH and evaluation of ?OH scavenging activities of antioxidants. |
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| Keywords: | hydroxyl radical liquid chromatography massspectrometry |
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