在线提取瓜子壳电喷雾技术在复杂基质样品检测中的应用

本研究建立了一种在线提取瓜子壳电喷雾检测装置，采用瓜子壳同时作为样品提取和喷雾装置，将样品提取和电喷雾质谱分析结合，被提取的成分直接进行喷雾检测，无需样品前处理。利用该方法分别对奶粉中三聚氰胺、土壤中莠去津、马铃薯中α-卡茄碱进行分析。三聚氰胺和莠去津的出峰良好，检测浓度可低至0.01 mg/kg，在高电场中瓜子壳实质是一个“电泳池”，带有不同电荷的物质向相反方向移动，使目标化合物从基质中解吸，被溶剂快速提取，获得较高的灵敏度。在马铃薯的不同位置取样检测α-卡茄碱，根据取样位置与信号强度做折线图，分析α-卡茄碱在马铃薯中的空间分布，发现在表皮发芽的部位信号最强，随着距离变远，信号强度逐渐减弱；其次，马铃薯内部的质谱信号强度降低速度比表面快，其质量检出限达到了0.001 g。该方法不仅可对多种复杂基质快速取样检测，在质谱成像方面也具有应用潜力。

Application of Online Extraction of Sunflower Seed Husk Electrospray Technology in Detection of Complex Matrix Samples

An online extraction of sunflower seed husk electrospray detection device was established. The sunflower seed husk was used as both sample extraction device and spray device. The sample extraction and electrospray mass spectrometry were combined organically, and the components in the sample were extracted and directly sprayed for detection without sample pretreatment, which greatly improved the detection efficiency. The melamine in milk powder, atrazine in soil, and α-caconine in potato were analyzed. The peaks of melamine and atrazine were good, and the lowest concentration detected reached 0.01 mg/kg. Because the sunflower seed husk was essentially an "electrophoresis pool" in a high electric field, and substances with different charges moved in the opposite direction, so that the target compound was desorbed from the matrix and was quickly extracted by the solvent, and this was the reason for its high sensitivity. The α-calconine in different positions of potato was detected, and a line graph was drawn according to the sampling position and signal intensity to analyze the spatial distribution of α-calconine in potato. It was found that the signal was the strongest in the sprouting area on the epidermis, and the signal strength gradually weakens as the distance became longer. Secondly, the signal intensity of the internal of potato decreased faster than the surface. The limit of detection reached 0.001 g. This method can not only quickly extract and detect target compounds of complex matrices samples, but also has application potential in mass spectrometry imaging.