| 光谱法研究异烟肼与牛血清白蛋白的相互作用 |
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| 引用本文： | 尚永辉,孙家娟,刘静.光谱法研究异烟肼与牛血清白蛋白的相互作用[J].现代科学仪器,2011(2):94-96. |
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| 作者姓名： | 尚永辉 孙家娟 刘静 |
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| 作者单位： | 咸阳师范学院化学与化工学院,陕西,咸阳,712000 |
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| 基金项目： | 国家自然科学基金项目(20975081); 陕西省教育厅科研计划项目(2010JK899); 西北大学研究生交叉学科资助项目(07YJC09) |
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| 摘 要： | 采用荧光光谱研究了异烟肼与牛血清白蛋白（BSA）的相互作用。异烟肼对BSA的荧光有较强的猝灭作用。根据研究292K，511K温度下异烟肼对BSA的荧光猝灭光谱，发现异烟肼对BSA的荧光猝灭属于动态猝灭过程，根据Frster非辐射能量转移理论计算出异烟肼与BSA间的结合距离r=5．60nm，结合常数（Kb）分别为5．；521X10^4 L·mol～（202K），5．189X104L·mol。（31lK），通过计算热力学数据焓变△rHm=-20．；545kJ·mol^-1，熵变△rSm=20．8J·mol^-1表明二者主要靠静电引力结合，采用同步荧光光谱探讨了异烟肼对BSA构象的影响。
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| 关 键 词： | 异烟肼 牛血清白蛋白 荧光光谱 |
Spectroscopic Study on Interaction of Isoniazid with Bovine Serum Albumin |
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| Shang Yonghui,Sun Jiajuan,Liu Jing.Spectroscopic Study on Interaction of Isoniazid with Bovine Serum Albumin[J].Modern Scientific Instruments,2011(2):94-96. |
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| Authors: | Shang Yonghui Sun Jiajuan Liu Jing |
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| Affiliation: | Shang Yonghui,Sun Jiajuan,Liu Jing(School of Chemistry & Chemical Engineering,Xianyang Normal University,Xianyang,Shaanxi 712000) |
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| Abstract: | The binding reaction of Isoniazid with bovine serum albumin was studied at 292K and 311K by fluorescence spectra. It was shown that Isoniazid has a strong ability of quenching the fluorescence of BSA, and the quenching mecha- nism of Isoniazid to BSA was a dynamic quenching. According to the Forster theory of non-radiation energy transfer, the binding distances （r） was 5.60 nm, the binding constants （Kb） were 5.321×104 L-mol^l（292K）, 3.189×104L.mol^1 （311K）, respectively. The thermodynamic parameters △rHm=-20.343 kJ·moll,ArSm=20.8 J·mol^1 showed that the interaction of Isoniazid and BSA is mainly driven by electrostatic force. Synchronous spectrum was used to investigate the conformational changes of BSA. |
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| Keywords: | Isoniazid Bovine Serum Albumin(BSA) Fluorescence spectroscopy |
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