| 用旋转式荧光定量PCR分析甲型H1N1流感病毒传播的特点 |
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| 引用本文： | 王安生,李洪敏,高华方,王思贤,赵智贤,杨楠.用旋转式荧光定量PCR分析甲型H1N1流感病毒传播的特点[J].现代科学仪器,2010(5):86-89. |
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| 作者姓名： | 王安生 李洪敏 高华方 王思贤 赵智贤 杨楠 |
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| 作者单位： | 1. 解放军第309医院结研所,北京,100091
2. 北京清华博奥生物有限公司,北京,100091 |
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| 基金项目： | 国家科技重大专项课题项目 |
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| 摘 要： | 目的：探讨旋转式荧光定量PcR,仪在检测临床标本中的应用价值。方法：通过旋转荧光定量-PUR技术对可疑病例的咽拭子、鼻拭子进行检测。结果2009年检测出甲流患者791例，检出率为；58．79％（791／20；59）；甲型H1N1157例，检出率为7．7％（157／2039），2010年1—6月检测出甲型H1N1为21例，检出率为1．44％（21／1455）。结论本地区甲型H1N1流感是以普通甲流感染为主，甲型H1N1流感混在其中，本地区的传播高峰是在2009年10月，甲型H1N1流感病毒在2010年来传播呈明显满弱始辞一
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| 关 键 词： | 甲型H1N1 Line—geneK—FQ—PcR 定量分析 |
Application of the Rotary Fluorescent Quantitative PCR in Epidemiology Characteristic of Influenza A/H1N1 |
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| Wang Ansheng,Li Hongmin,Gao Huafang,Wang Sixian,Zhao Zhixian,Yang Nan.Application of the Rotary Fluorescent Quantitative PCR in Epidemiology Characteristic of Influenza A/H1N1[J].Modern Scientific Instruments,2010(5):86-89. |
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| Authors: | Wang Ansheng Li Hongmin Gao Huafang Wang Sixian Zhao Zhixian Yang Nan |
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| Affiliation: | 1Tuberculosis Research Center, the 309th Hospital of the PLA, China, Beijing 100091, P.R.China; 2Corporation Technology Corporation Co. Ltd, Beijing 100091,China) |
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| Abstract: | Objective： The application of rotary fluorescent quantitative PCR instrument in clinical detection. Method： Pharynx and nose swabs of 3494 doubtful cases were detcted with Line-geneK-FQ-PCR. Results： Influenza A patients were 791, and detection rate was 38.79%（791/2039）. Patients who in fected H 1N 1 virus were 157, and detection rate was 7.7%（157/2039）. Patients who infected H1N1 virus were 21 in 2010.l-2010.6, and detection rate was 1.44%（21/1455）. Conclusion： Spreading peak of influenza A/H 1N 1 occurred in October 2009. Transmiting tendency obviously weakened from 2010. |
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| Keywords: | H1N1 Line-geneK-FQ-PCR Quantitative analysis |
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