发光二极管诱导荧光微芯片分析检测器的研制

以高功率发光二极管(LED)为激发光源,研制了一种小型LED诱导荧光检测器,用于微流控芯片分析检测.利用MOS管压控恒流原理,设计恒流驱动电路,使高功率LED发光稳定.通过设计和制造光学结构,成功地将LED发散光聚焦成约3.5 mm×0.3 mm的线状光束,实现了与微流控芯片中的微通道对准,简化了复杂的机械校准结构,且大大减少了光学系统体积(约为9.5 cm×4 cm×17 cm).用荧光染料NBD和高密度脂蛋白评价该体系的性能,结果表明,LED激发光源稳定,检测器重复性好,可用于微流控芯片毛细管电泳分析检测,基本实现了LED诱导荧光检测器的微型化.

High-power light-emitting-diode induced fluorescence detector for microfluidic chip analysis

A novel miniaturized fluorescence detector by using a blue high-power Light-emitting-Diode(LED) as its excitation light source was developed for microfluidic chip analysis. Based on the constant current principle of MOS tubes,a constant current driving circuit was designed to control the LED to generate a stable light.A special optical structure was designed and manufacturized to focus the divergent light emitted by the LED into a linear beam with the size about 3.5 mm×0.3 mm. This linear beam could be easily aligned to the microchannel of a microfluidic chips to reduce the complexity of the detector and miniaturize the size of the detector, and the bulk of the optical system was about 9.5 cm×4 cm×17 cm. NBD C6-ceramide and high density lipoprotein were used to evaluate the performance of the detector, the results indicate that the LED excitation source is stable,and the detector has good reproducibility and can be used in the capillary electrophoresis analysis of microfluidic chips.