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| 用于阵列样品检测的扫描式表面等离子体共振生物传感器 | |
| 引用本文： | 齐攀,李莹,冯明创,钟金钢.用于阵列样品检测的扫描式表面等离子体共振生物传感器[J].光学精密工程,2012,20(11):2365-2372. |
| 作者姓名： | 齐攀 李莹 冯明创 钟金钢 |
| 作者单位： | 1. 暨南大学光电工程系,广东广州510632;广东交通职业技术学院电子工程系,广东广州510650 2. 暨南大学光电信息与传感技术广东普通高校重点实验室,广东广州,510632 3. 暨南大学光电工程系,广东广州510632;暨南大学光电信息与传感技术广东普通高校重点实验室,广东广州510632 |
| 基金项目： | 海洋赤潮灾害立体监测技术与应用国家海洋局重点实验室资助项目(No.MATHAB20120208)；暨南大学优秀本科推免生科研创新培育计划资助项目；粤港关键领域重点突破招标资助项目(No.2005A20501001) |
| 摘 要： | 针对阵列样品的定量检测,构建了一种用于阵列样品检测的扫描式表面等离子体共振生物传感器。首先,基于平面棱镜耦合下的最佳旋转轴位置和双棱镜探测光路搭建了阵列扫描式表面等离子体共振生物传感器。然后,计算了可探测阵列样品的点密度。最后,以蒸馏水和浓度分别为5,10,15,20,25,30,35mg/mL的葡萄糖溶液作为待测阵列样品进行了多样品点的表面等离子体共振实验。实验测得阵列样品的共振角分别为73.745,73.919,74.052,74.185,74.306,74.408,74.549,74.660°,显示葡萄糖溶液浓度与共振角线性关系良好,证明了该方法和装置的可行性。该装置对提高阵列样品点密度,实现高精度定量检测具有重要意义。 |
| 关 键 词： | 表面等离子体共振 生物传感器 阵列扫描 样品密度 |
| 收稿时间： | 2012/5/25 |
Scanning surface plasmon resonance biosensor for array sample detection |
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| QI Pan , LI Ying , FENG Ming-chuang , ZHONG Jin-gang.Scanning surface plasmon resonance biosensor for array sample detection[J].Optics and Precision Engineering,2012,20(11):2365-2372. | |
| Authors: | QI Pan LI Ying FENG Ming-chuang ZHONG Jin-gang |
| Affiliation: | 1,3(1.Department of Optoelectronic Engineering,Jinan University,Guangzhou 510632,China; 2.Department of Electronic Engineering,Guangdong Communication Polytechnic Institute, Guangzhou 510650,China; 3.Laboratory of Optoelectronic Information and Sensing Technologies of Guangdong Higher Education Institute,Jinan University,Guangzhou 510632,China) |
| Abstract: | An scanning surface plasmon resonance biosensor was constructed to quantitatively detect array samples. First, based on the optimal rotation position in plane prism-coupling mode and a double-prism detection optical path, the scanning surface plasmon resonance biosensor for array samples was constructed. Then, the sample density of array samples was calculated. Finally, by taking the distilled water and glucose solution with different concentrations in 5, 10, 15, 20, 25, 30 and 35 mg/mL as array samples to be measured, the multi-sample spots were detected by surface plasmon resonance method. Experimental results show that the resonance angles of array samples are 73.745, 73.919, 74.052, 74.185, 74.306, 74.408, 74.549 and 74.660°, respectively, and the solution concentrations and their resonance angles show a good linear relation, which proves that the proposed method and device are feasibility. The device is significant for increasing the sample density of array samples and realizing the high precision detection. |
| Keywords: | surface plasmon resonance biosensor array scanning sample density |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |