| 高效液相色谱法测定胡黄连及清热八味散中阿魏酸 |
| |
| 引用本文： | 尹德忠,赵红侠,吴耀国,刘建勋.高效液相色谱法测定胡黄连及清热八味散中阿魏酸[J].现代仪器,2007,13(3):39-40,49. |
| |
| 作者姓名： | 尹德忠 赵红侠 吴耀国 刘建勋 |
| |
| 作者单位： | 1. 西北工业大学理学院,西安,710072
2. 陕西诚信制药有限公司,渭南,715200 |
| |
| 摘 要： | 本文建立反相高效液相色谱法测定胡黄连药材及其制剂清热八味散中阿魏酸含量的方法。采用C18-ODS柱,乙腈-0.05%磷酸溶液(15∶85)为流动相;313nm检测。阿魏酸线性范围52.0~520μg,r=0.9995,胡黄连药材和清热八味散平均回收率分别为98.85%和98.32%,含量测定重复性RSD分别为1.4%和1.5%。方法准确,重复性好,为制定胡黄连药材及其制剂的质量标准提供参考依据。
|
| 关 键 词： | 高效液相色谱法 胡黄连 清热八味散 阿魏酸 |
Determination of ferulic acid in Picrohiza scrophulariiflora Pennell and Qingre Bawei San by HPLC |
| |
| Yin Dezhong,Zhao Hongxia,Wu Yaoguo,Liu Jianxun.Determination of ferulic acid in Picrohiza scrophulariiflora Pennell and Qingre Bawei San by HPLC[J].Modern Instruments,2007,13(3):39-40,49. |
| |
| Authors: | Yin Dezhong Zhao Hongxia Wu Yaoguo Liu Jianxun |
| |
| Abstract: | A RP-HPLC method was described for determination of ferulic acid in Picrohiza scrophulariiflora Pennell and its preparation Qingre Bawei San.C18-ODS column was used with a mobile phase of acetonitrile-0.05% phosphoric acid(15:85) and the detection wavelength was 313nm.The calibration curve was linear in the range of 52.0 ～520μg ferulic acid(r=0.9995).The average recovery was 98.85% and 98.32%,the RSD of reproducibility was 1.4% and 1.5% for Picrohiza scrophulariiflora Pennell and Qingre Bawei San respectively.The method was accurate with a good reproducibility and provided basis for developing quality control method for Picrohiza scrophulariiflora Pennell and its preparation. |
| |
| Keywords: | HPLC Picrohiza scrophulariiflora Pennell Qingre Bawei San Ferulic acid |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
