| HPLC法测定甲磺酸帕珠沙星中光学异构体的方法研究 |
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| 引用本文： | 蔡振华,李月鹏,蒋庆峰.HPLC法测定甲磺酸帕珠沙星中光学异构体的方法研究[J].现代仪器,2005,11(6):23-24. |
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| 作者姓名： | 蔡振华 李月鹏 蒋庆峰 |
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| 作者单位： | 天津药物研究院分析测试中心,天津,300193 |
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| 摘 要： | 采用HPLC法测定甲磺酸帕珠沙星中的光学异构体。采用色谱柱Inertsil ODS-35μm 4.6×250mm;流动相为乙腈:0.2mol/L无水柠檬酸(pH3.0):水(每100 mL水中加D-苯丙胺酸0.15g,加氯化铜0.15g溶解后混匀)=8∶1∶92;检测波长322nm,流速1.5mL/min。在0.10~0.30μg范围内,主药峰面积与进样量线性关系良好,r=0.9999。用HPLC法测定甲磺酸帕珠沙星中光学异构体,方法简便,专属性及重现性好。
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| 关 键 词： | HPLC 甲磺酸帕珠沙星 光学异构体 |
Sepecation of pazufloxacin mesilate and its optical isomer by HPLC |
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| Cai Zhenhua,Li Yuepeng,Jiang Qingfeng.Sepecation of pazufloxacin mesilate and its optical isomer by HPLC[J].Modern Instruments,2005,11(6):23-24. |
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| Authors: | Cai Zhenhua Li Yuepeng Jiang Qingfeng |
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| Abstract: | Objective A HPLC method was established for the determination of Pazufloxacin mesilate and its optical isomer.The HPLC system consisted of Inertsil ODS-3 Column(Diamonsil 4.6 mm×250mm,5um) with the mobile phase of acetonitrile:0.2moL·L~(-1)anhydrous citric acid(pH3.0) :water(D-phenylalanine0.15g and cupric chloride 0.15g ·100moL ~(-1))=(8∶1∶92).The flow rate was 1.5mL·min~(-1) and detected at 322nm.The standard curve was linear in the range of 0.10~0.30μg,and the correlation coefficient is 0.9999.The proposed method is convenience,selective and reproducible,and can be used to determination of Pazufloxacin mesilate and its optical isomer. |
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| Keywords: | HPLC Pazufloxacin mesilate Optical isomer |
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