Scanning electron microscopy of Streptomyces without use of any chemical fixatives |
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Authors: | Kumar Vijay Bharti Alpana Gusain Omprakash Bisht Gajraj Singh |
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Affiliation: | Department of Microbiology, Sardar Bhagwan Singh Post Graduate Institute of Biomedical Sciences and Research, Balawala, Dehradun, Uttarakhand, India. |
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Abstract: | A new, short, and quick method was developed for preparation of specimen for observing Actinomycetes of genus Streptomyces by scanning electron microscopy. The cultures were directly grown on stubs and coated with a film of gold without using any fixative and dehydrating procedures. Using this simple preparation procedure, surface of intact sporing structures of Streptomyces was observed over a range of magnifications. As the preparation procedure is so simple and rapid, this procedure could be most useful for the routine examination and identification of Streptomyces. |
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Keywords: | fixatives scanning stubs Streptomyces |
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