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| 基因芯片荧光靶点显微成像自动调焦控制 | |
| 引用本文： | 刘军,冯艳君,刘伟军,王天然.基因芯片荧光靶点显微成像自动调焦控制[J].仪表技术与传感器,2011(5). |
| 作者姓名： | 刘军 冯艳君 刘伟军 王天然 |
| 作者单位： | 1. 沈阳理工大学信息科学与工程学院,辽宁沈阳110159;中国科学院沈阳自动化研究所,辽宁沈阳110016 2. 沈阳理工大学信息科学与工程学院,辽宁沈阳,110159 3. 中国科学院沈阳自动化研究所,辽宁沈阳,110016 |
| 摘 要： | 在基因芯片荧光靶点阵列图像CCD扫描采集系统中,显微成像自动调焦控制是进行芯片杂交信号荧光靶点图像采集的先决条件.在定义图像清晰度评价函数的基础上,通过对实时采集的显微图像进行清晰度评价函数计算,采用基于粗精结合原则的自动调焦控制策略,实现了荧光靶点显微成像自动调焦控制.调焦控制实验表明,该方法显著提高了基因芯片荧光靶点图像聚焦和采集的效率. |
| 关 键 词： | 自动调焦控制 基因芯片 显微成像 图像处理 清晰度评价函数 |
Auto-focus Control for Microscopic Imaging of Gene-chip Fluorescent Spots |
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| LIU Jun,FENG Yan-jun,LIU Wei-jun,WANG Tian-ran.Auto-focus Control for Microscopic Imaging of Gene-chip Fluorescent Spots[J].Instrument Technique and Sensor,2011(5). | |
| Authors: | LIU Jun FENG Yan-jun LIU Wei-jun WANG Tian-ran |
| Affiliation: | LIU Jun1,2,FENG Yan-jun1,LIU Wei-jun2,WANG Tian-ran2(1.School of Information Science and Engineering,Shenyang Ligong University,Shenyang 110159,China,2.Shenyang Institute of Automation,Chinese Academy of Sciences,Shenyang 110016,China) |
| Abstract: | In gene-chip micro-array fluorescent spots images CCD scanning system,microscopic auto-focusing control is the prerequisite task for micro-array hybridized fluorescent spots images acquiring.Based on the definitions for image clarity evaluating functions,this paper computed the clarity evaluating functions for the acquired defocus and focus images in real-time,and then it employed a microscopic auto-focusing strategy according to the coarse and fine focusing criterion to realize auto-focusing control for th... |
| Keywords: | auto-focus control gene-chip microscopic imaging image processing evaluation function |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |