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榆耳菌丝体多糖提取及脱色条件的研究
引用本文:张公亮,王应男,刘洋,张浩,侯红漫. 榆耳菌丝体多糖提取及脱色条件的研究[J]. 食品科学, 2012, 33(24): 147-151. DOI: 10.7506/spkx1002-6630-201224030
作者姓名:张公亮  王应男  刘洋  张浩  侯红漫
作者单位:大连工业大学食品学院,辽宁 大连 116034
摘    要:对榆耳菌丝体多糖提取条件和脱色条件进行研究,以提高产品的得率和纯度。通过单因素及正交试验,得到榆耳菌丝体胞内多糖的最佳提取条件:水料比40:1、提取时间8h、提取温度95℃、提取次数3次。在此条件下,榆耳菌丝体胞子内多糖得率为8.60%。采用AB-8大孔树脂对多糖脱色,最佳条件为添加量0.2g/mL、pH4.0、温度60℃。

关 键 词:榆耳  菌丝体  多糖  提取  脱色  
收稿时间:2011-09-24

Extraction and Decolorization of Mycelial Polysaccharides from Gloeostrereum incarnatum
ZHANG Gong-liang,WANG Ying-nan,LIU Yang,ZHANG Hao,HOU Hong-man. Extraction and Decolorization of Mycelial Polysaccharides from Gloeostrereum incarnatum[J]. Food Science, 2012, 33(24): 147-151. DOI: 10.7506/spkx1002-6630-201224030
Authors:ZHANG Gong-liang  WANG Ying-nan  LIU Yang  ZHANG Hao  HOU Hong-man
Affiliation:School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China
Abstract:Orthogonal array design was used to optimize conditions for the extraction and de-colorization of mycelial polysaccharides from Gloeostereum incarnatum to obtain enhanced yield and purity. The optimum extraction conditions were found to be three extraction cycles at 95 ℃ for 8 h with a water-to-solid ratio of 40:1. Under these conditions, the polysaccharide yield was 8.60%. AB-8 resin was used to decolorize the crude polysaccharide extract, and the optimum decolorization conditions were found to be a solid-to-liquid ratio of 0.2 g/mL, pH 4.0 and 60 ℃.
Keywords:Gloeostereum insarnatum  mycelium  polysaccharides  extraction  de-colorization  
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